AADAT cDNA Clone | MyBioSource MBS1270390 product information

product summary

company name :

MyBioSource

product type :

cDNA

product name :

AADAT cDNA Clone

catalog :

MBS1270390

quantity :

10 ug Plasmid + 200

price :

175 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS1270390

products type :

cDNA Clone

products full name :

AADAT cDNA Clone

products short name :

AADAT

other names :

Homo sapiens aminoadipate aminotransferase, mRNA; Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial; kynurenine/alpha-aminoadipate aminotransferase, mitochondrial; KAT/AadAT; 2-aminoadipate transaminase; kynurenine aminotransferase II; 2-aminoadipate aminotransferase; kynurenine--oxoglutarate transaminase 2; kynurenine--oxoglutarate transaminase II; kynurenine--oxoglutarate aminotransferase II; L kynurenine/alpha aminoadipate aminotransferase; aminoadipate aminotransferase; 2-aminoadipate aminotransferase; 2-aminoadipate transaminase (EC:2.6.1.39); Alpha-aminoadipate aminotransferase; AadAT; Kynurenine aminotransferase II; Kynurenine--oxoglutarate aminotransferase II; Kynurenine--oxoglutarate transaminase 2 (EC:2.6.1.7); Kynurenine--oxoglutarate transaminase II

products gene name :

AADAT

other gene names :

AADAT; AADAT; KAT2; KATII; KAT2; KAT/AadAT; AadAT

uniprot entry name :

AADAT_HUMAN

sequence length :

2128

sequence :

atgaattacgcacggttcatcacggcagcgagcgcagcc
agaaacccttctcccatccggaccatgagtgagaaacgg
gctgacatattgagcagaggaccaaaatcgatgatctcc
ttggctggtggcttaccaaatccaaacatgtttcctttt
aagactgccgtaatcactgtagaaaatggaaagaccatc
caatttggagaagagatgatgaagagagcacttcagtat
tctccgagtgctggaattccagagcttttgtcctggcta
aaacagttacaaataaaattgcataatcctcctaccatc
cattaccaacccagtcaaggacaaatggatctatgtgtc
acatctggcagccaacaaggtctttgtaaggtgtttgaa
atgatcattaatcctggagataatgtcctcctagatgaa
cctgcttattcaggaactcttcaaagtctgcacccactg
ggctgcaacattattaatgttgccagtgatgaaagtggg
attgttccagattccctaagagacatactttccagatgg
aaaccagaagatgcaaagaatccccagaaaaacaccccc
aaatttctttatactgttccaaatggcaacaaccctact
ggaaactcattaaccagtgaacgcaaaaaggaaatctat
gagcttgcaagaaaatatgatttcctcataatagaagat
gatccttactattttctccagtttaacaagttcagggta
ccaacatttctttccatggatgttgatggacgtgtcatc
agagctgactctttttcaaaaatcatttcctctgggttg
agaataggatttttaactggtccaaaacccttaatagag
agagttattttacacatacaagtttcaacattgcacccc
agcacttttaaccagctcatgatatcacagcttctacac
gaatggggagaagaaggtttcatggctcatgtagacagg
gttattgatttctatagtaaccagaaggatgcaatactg
gcagctgcagacaagtggttaactggtttggcagaatgg
catgttcctgctgctggaatgtttttatggattaaagtt
aaaggcattaatgatgtaaaagaactgattgaagaaaag
gccgttaagatgggggtattaatgctccctggaaatgct
ttctacgtcgatagctcagctcctagcccttacttgaga
gcatccttctcttcagcttctccagaacagatggatgtg
gccttccaggtattagcacaacttataaaagaatcttta
tga

other info1 :

Vector: pENTR223.1

ncbi gi num :

21410575

ncbi acc num :

BC031068

uniprot acc num :

Q8N5Z0

ncbi mol weight :

47,352 Da

ncbi pathways :

2-Oxocarboxylic Acid Metabolism Pathway 714485!!2-Oxocarboxylic Acid Metabolism Pathway 717400!!Biosynthesis Of Amino Acids Pathway 790012!!Biosynthesis Of Amino Acids Pathway 795174!!Lysine Biosynthesis Pathway 82955!!Lysine Biosynthesis Pathway 319!!Lysine Catabolism Pathway 106187!!Lysine Degradation Pathway 82956!!Lysine Degradation Pathway 320!!Lysine Degradation, Lysine = Saccharopine = Acetoacetyl-CoA Pathway 413352

ncbi summary :

This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro). Ref.5. Catalytic activity: L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate. Ref.2 Ref.5 Ref.6L-2-aminoadipate + 2-oxoglutarate = 2-oxoadipate + L-glutamate. Ref.2 Ref.5 Ref.6. Cofactor: Pyridoxal phosphate. Ref.2 Ref.6 Ref.7. Enzyme regulation: Kynurenine transaminase activity is competitively inhibited by aminoadipate, asparagine, glutamate, histidine, cysteine, lysine, 3-hydroxy-kynurenine and phenylalanine. Ref.5. Pathway: Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 4/6. Subunit structure: Homodimer. Ref.5 Ref.6 Ref.7. Subcellular location: Mitochondrion . Potential. Tissue specificity: Higher expression in the liver. Also found in heart, brain, kidney, pancreas, prostate, testis and ovary. Sequence similarities: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Biophysicochemical propertiesKinetic parameters:KM=0.9 mM for aminoadipate Ref.5KM=4.7 mM for kynurenineKM=1.7 mM for methionineKM=1.6 mM for glutamateKM=1.8 mM for tyrosineKM=1.2 mM for 2-oxoglutarateKM=1.5 mM for 2-oxocaproic acidKM=1.8 mM for phenylpyruvateKM=1.4 mM for ino-3-pyruvatepH dependence:Optimum pH is 7-9.Temperature dependence:Optimum temperature is 50 degrees Celsius.

size :

10 ug Plasmid + 200 ul Glycerol Stock

price :

175 USD

more info or order :

MyBioSource product webpage