NOX4 Polyclonal Antibody | MyBioSource MBS127001 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

NOX4 Polyclonal Antibody

catalog :

MBS127001

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using NOX4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

image 2 :

Immunofluorescence analysis of HeLa cells using NOX4 antibody. Blue: DAPI for nuclear staining.

product information

catalog number :

MBS127001

products type :

Antibody

products full name :

NOX4 Polyclonal Antibody

products short name :

[NOX4]

products name syn :

[KOX; KOX-1; RENOX]

other names :

[NADPH oxidase 4; NADPH oxidase 4; NADPH oxidase 4; kidney oxidase-1; renal NAD(P)H-oxidase; kidney superoxide-producing NADPH oxidase; NADPH oxidase 4; Kidney oxidase-1; KOX-1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase]

products gene name :

[NOX4]

other gene names :

[NOX4; NOX4; KOX; KOX-1; RENOX; RENOX; KOX-1]

uniprot entry name :

NOX4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

578

purity :

Affinity Purification

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF)

app notes :

WB: 1:500 - 1:2000. IF 1:10-1:100

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 444-578 of human NOX4 (NP_058627.1). Calculated MW: 6kDa/25-31kDa/58-66kDa. Observed MW: 67 kDa

products categories :

Polyclonal

products description :

NOX4 (NADPH oxidase 4) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and it has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex.NOX4 has 7 isforms and the molecular weight are 7KDa,26-32KDa,58-67KDa,75-80KDa(PMID:11728818).This antibody is specific to NOX4.

ncbi gi num :

212276447

ncbi acc num :

Q9NPH5.2

uniprot acc num :

Q9NPH5

ncbi pathways :

Oxidative Stress Pathway (198916); Signaling Events Mediated By PTP1B Pathway (138017); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

uniprot summary :

NOX4: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; EC 1.6.3.-; Membrane protein, multi-pass; Cell cycle regulation; Nucleolus; Oxidoreductase. Chromosomal Location of Human Ortholog: 11q14.2-q21. Cellular Component: endoplasmic reticulum membrane; focal adhesion; mitochondrion; perinuclear region of cytoplasm; apical plasma membrane; nucleolus; integral to membrane; stress fiber; NADPH oxidase complex. Molecular Function: oxygen sensor activity; electron carrier activity; FAD binding; NAD(P)H oxidase activity; superoxide-generating NADPH oxidase activity; nucleotide binding; heme binding. Biological Process: positive regulation of apoptosis; cell morphogenesis; cell aging; homocysteine metabolic process; positive regulation of smooth muscle cell migration; cardiac muscle cell differentiation; positive regulation of MAP kinase activity; negative regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of stress fiber formation; response to hypoxia; inflammatory response; superoxide release; bone resorption

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage