HNF4A Polyclonal Antibody | MyBioSource MBS126772 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

HNF4A Polyclonal Antibody

catalog :

MBS126772

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using HNF4A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

image 2 :

Immunohistochemistry of paraffin-embedded rat pancreas using HNF4A Antibody at dilution of 1:100 (40x lens).

image 3 :

Immunohistochemistry of paraffin-embedded mouse liver using HNF4A Antibody at dilution of 1:100 (40x lens).

product information

catalog number :

MBS126772

products type :

Antibody

products full name :

HNF4A Polyclonal Antibody

products short name :

[HNF4A]

products name syn :

[FRTS4; HNF4A; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14]

other names :

[Hepatocyte nuclear factor 4-alpha; Hepatocyte nuclear factor 4-alpha; hepatocyte nuclear factor 4-alpha; TCF-14; HNF4alpha10/11/12; transcription factor 14; transcription factor HNF-4; hepatic nuclear factor 4 alpha; nuclear receptor subfamily 2 group A member 1; hepatocyte nuclear factor 4, alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4]

products gene name :

[HNF4A]

other gene names :

[HNF4A; HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha; HNF4; NR2A1; TCF14; HNF-4-alpha; TCF-14]

uniprot entry name :

HNF4A_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

474

purity :

Affinity Purification

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

WB, IHC

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Immunohistochemistry of paraffin-embedded rat liver using HNF4A Antibody at dilution of 1:100 (40x lens).

image5 heading :

Immunohistochemistry (IHC)

image5 description :

Immunohistochemistry of paraffin-embedded rat pancreas using HNF4A Antibody at dilution of 1:100 (40x lens).

other info1 :

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

other info2 :

Immunogen: A synthetic peptide of human HNF4A. Calculated MW: 43-56kDa. Observed MW: 53 kDa

products categories :

Polyclonal

products description :

Hepatocyte nuclear factor 4alpha (HNF4alpha) is a transcription factor that belongs to the steroid hormone receptor superfamily and is enriched in liver (1). HNF4alpha, in association with PGC-1alpha, activates gluconeogenic genes such as phosphoenolpyruvate carboxykinase and glucose-6-phosphatase genes in fasted livers (2,3). Conditional knockout of the HNF4alpha gene in the mouse liver destroys lipid homeostasis and leads to lipid accumulation in the liver and a reduction of serum cholesterol and triglyceride levels (4). Mutations in HNF4alpha have been linked to maturity-onset diabetes of the young (MODY) (5).

ncbi gi num :

148886624

ncbi acc num :

P41235.3

uniprot acc num :

P41235

ncbi pathways :

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Developmental Biology Pathway (477129); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); HIF-1-alpha Transcription Factor Network Pathway (138045); Integrated Pancreatic Cancer Pathway (711360); Maturity Onset Diabetes Of The Young Pathway (83096)

ncbi summary :

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]

uniprot summary :

HNF4 alpha: a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Four alternatively spliced isoforms have been described. Protein type: Nuclear receptor; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: nucleoplasm; cytoplasm; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor activity; protein binding; protein homodimerization activity; DNA binding; zinc ion binding; steroid hormone receptor activity; transcription factor activity; fatty acid binding; receptor binding. Biological Process: transcription initiation from RNA polymerase II promoter; regulation of lipid metabolic process; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; lipid homeostasis; glucose homeostasis; endocrine pancreas development; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; xenobiotic metabolic process; regulation of gastrulation; response to glucose stimulus; ornithine metabolic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; lipid metabolic process; negative regulation of cell growth; blood coagulation; regulation of insulin secretion; sex differentiation; phospholipid homeostasis. Disease: Fanconi Renotubular Syndrome 4 With Maturity-onset Diabetes Of The Young; Maturity-onset Diabetes Of The Young, Type 1; Diabetes Mellitus, Noninsulin-dependent

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage