EEF1A1 Polyclonal Antibody | MyBioSource MBS126370 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

EEF1A1 Polyclonal Antibody

catalog :

MBS126370

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using EEF1A1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

image 2 :

Immunofluorescence analysis of HeLa cells using EEF1A1 antibody. Blue: DAPI for nuclear staining.

product information

catalog number :

MBS126370

products type :

Antibody

products full name :

EEF1A1 Polyclonal Antibody

products short name :

[EEF1A1]

products name syn :

[EEF1A1; CCS-3; CCS3; EEF-1; EEF1A; EF-Tu; EF1A; FLJ25721; GRAF-1EF; HNGC:16303; LENG7; MGC102687; MGC131894; MGC16224; PTI1; eEF1A-1]

other names :

[Elongation factor 1-alpha 1; Elongation factor 1-alpha 1; elongation factor 1-alpha 1; EF-1-alpha-1; EF1a-like protein; CTCL tumor antigen; elongation factor Tu; cervical cancer suppressor 3; elongation factor 1 alpha subunit; prostate tumor-inducing protein 1; eukaryotic elongation factor 1 A-1; leukocyte receptor cluster member 7; leukocyte receptor cluster (LRC) member 7; translation elongation factor 1 alpha 1-like 14; glucocorticoid receptor AF-1 specific elongation factor; eukaryotic translation elongation factor 1 alpha 1-like 14; eukaryotic translation elongation factor 1 alpha 1; Elongation factor Tu; EF-Tu; Eukaryotic elongation factor 1 A-1; eEF1A-1; Leukocyte receptor cluster member 7]

products gene name :

[EEF1A1]

other gene names :

[EEF1A1; EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303; EEF1A; EF1A; LENG7; EF-1-alpha-1; EF-Tu; eEF1A-1]

uniprot entry name :

EF1A1_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

unknown

reactivity :

Human, Mouse, Rat

sequence length :

462

purity :

Affinity Purification

storage stability :

Store at -20°C. Avoid freeze/thaw cycles.

tested application :

WB, IF

app notes :

WB: 1:500 - 1:2000. IF: 1:20 - 1:100

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

. Source: Rabbit. Buffer: PBS with 0.02% sodium azide, 50% glycerol,pH 7.3

other info2 :

Immunogen: Recombinant protein of human EEF1A1. Calculated Molecular Weight: 47kDa/50kDa. Observed MW: 50kDa

products categories :

Polyclonal

products description :

Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells (1). eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and interacts with amino acyl-tRNAs to promote recruitment of amino acyl-tRNAs to the A-site of the ribosome (1). After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B (1). Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level (2,3).

ncbi gi num :

55584035

ncbi acc num :

P68104.1

uniprot acc num :

P68104

ncbi pathways :

Cellular Response To Heat Stress Pathway (1127553); Cellular Responses To Stress Pathway (645258); EGFR1 Signaling Pathway (198782); Eukaryotic Translation Elongation Pathway (105976); Gene Expression Pathway (105937); HSF1 Activation Pathway (1127554); Legionellosis Pathway (469200); Legionellosis Pathway (469186); Metabolism Of Proteins Pathway (106230); Peptide Chain Elongation Pathway (105977)

ncbi summary :

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]

uniprot summary :

EEF1A1: an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Protein type: Nucleolus; Translation; Translation elongation. Chromosomal Location of Human Ortholog: 6q14.1. Cellular Component: extracellular space; cortical actin cytoskeleton; eukaryotic translation elongation factor 1 complex; membrane; cytoplasm; nucleolus; nucleus; cytosol. Molecular Function: GTPase activity; protein binding; GTP binding; protein kinase binding; translation elongation factor activity; tRNA binding. Biological Process: cellular protein metabolic process; translational elongation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; translation; gene expression

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage