POT1 Polyclonal Antibody | MyBioSource MBS126278 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

POT1 Polyclonal Antibody

catalog :

MBS126278

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using POT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

image 2 :

Immunohistochemistry of paraffin-embedded rat kidney using POT1 antibody at dilution of 1:100 (40x lens).

image 3 :

Immunohistochemistry of paraffin-embedded mouse lung using POT1 antibody at dilution of 1:100 (40x lens).

product information

catalog number :

MBS126278

products type :

Antibody

products full name :

POT1 Polyclonal Antibody

products short name :

[POT1]

products name syn :

[CMM10;GLM9;HPOT1]

other names :

[Protection of telomeres protein 1; Protection of telomeres protein 1; protection of telomeres protein 1; protection of telomeres 1 homolog; POT1-like telomere end-binding protein; protection of telomeres 1; POT1-like telomere end-binding protein]

products gene name :

[POT1]

other gene names :

[POT1; POT1; CMM10; HPOT1; hPot1]

uniprot entry name :

POTE1_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

unknown

reactivity :

Human, Mouse, Rat

sequence length :

634

purity :

Affinity Purification

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:200 - 1:2000. IHC: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Immunohistochemistry of paraffin-embedded mouse liver using POT1 antibody at dilution of 1:100 (40x lens).

image5 heading :

Immunohistochemistry (IHC)

image5 description :

Immunohistochemistry of paraffin-embedded mouse spleen using POT1 antibody at dilution of 1:100 (40x lens).

other info1 :

Source: Rabbit. Species: Human

other info2 :

Immunogen: Fusion protein of human POT1. Calculated Molecular Weight: 71kDa. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products categories :

Polyclonal

products description :

Protection of telomeres 1(POT1)is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase.

ncbi gi num :

50401179

ncbi acc num :

Q9NUX5.1

uniprot acc num :

Q9NUX5

ncbi pathways :

Cell Cycle Pathway (530733); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chromosome Maintenance Pathway (161048); DNA Damage/Telomere Stress Induced Senescence Pathway (905994); Meiosis Pathway (477133); Meiotic Synapsis Pathway (477134); Packaging Of Telomere Ends Pathway (106548); Regulation Of Telomerase Pathway (137987); Shelterin Complex Pathway (470348)

ncbi summary :

This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

POT1: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA- binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5 -TTAGGG-3 repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5 - TAGGGTTAG-3 sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3 -end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity. Belongs to the telombin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 7q31.33. Cellular Component: nucleoplasm; chromosome, telomeric region; nuclear telomere cap complex; cytoplasm; nucleus. Molecular Function: protein binding; DEAD/H-box RNA helicase binding; single-stranded telomeric DNA binding. Biological Process: positive regulation of helicase activity; negative regulation of telomerase activity; telomere assembly; telomere capping; telomere maintenance via telomerase; positive regulation of telomerase activity; negative regulation of telomere maintenance via telomerase; telomere maintenance; DNA duplex unwinding; positive regulation of telomere maintenance via telomerase. Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 10

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage