Histone H3K36me1 Polyclonal Antibody | MyBioSource MBS126245 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Histone H3K36me1 Polyclonal Antibody

catalog :

MBS126245

quantity :

0.05 mL

price :

235 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K36 antibody (MBS126245). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

image 2 :

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K36 antibody.

image 3 :

Immunohistochemistry of paraffin-embedded rat brain using MonoMethyl-Histone H3-K36 antibody at dilution of 1:200 (40x lens).

product information

catalog number :

MBS126245

products type :

Antibody

products full name :

Histone H3K36me1 Polyclonal Antibody

products short name :

[H3K36me1]

products name syn :

[HIST3H3; H3.4; H3/g; H3FT; H3t; histone H3.1t]

other names :

[Histone H3.1t; Histone H3.1t; histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T; histone cluster 3, H3; H3/g]

products gene name :

[H3K36me1]

other gene names :

[HIST3H3; HIST3H3; H3t; H3.4; H3/g; H3FT; H3FT; H3/t; H3t]

uniprot entry name :

H31T_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

rabbit

reactivity :

Human, Mouse, Rat, Other (Wide Range)

sequence length :

136

purity :

Affinity Purification

concentration :

1mg/ml

storage stability :

Store at -20 degree C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP, CHIPseq

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200. IP: 1:50 - 1:200. ChIP: 1:20 - 1:100. CHIPseq: 1:20-1:100

image1 heading :

Western Blot (WB)

image2 heading :

Dot Blot (DB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunofluorescence (IF)

image4 description :

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K36 antibody. Blue: DAPI for nuclear staining.

image5 heading :

Chromatin immunoprecipitation (ChIP)

image5 description :

Chromatin immunoprecipitation analysis extracts of 293 cell line, using MonoMethyl-Histone H3-K36 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

other info1 :

Category: Methylated Antibodies

other info2 :

Immunogen: A synthetic methylated peptide corresponding to residues surrounding K36 of human histone H3. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

products categories :

Methylation

products description :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

ncbi gi num :

18202512

ncbi acc num :

Q16695.3

uniprot acc num :

Q16695

ncbi mol weight :

Calculated MW: 15kDa. Observe MW: 16kDa

ncbi pathways :

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chromosome Maintenance Pathway (161048); Condensation Of Prophase Chromosomes Pathway (1127547); DNA Damage/Telomere Stress Induced Senescence Pathway (905994); Disease Pathway (530764)

ncbi summary :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

uniprot summary :

HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 1q42. Cellular Component: nucleoplasm; nucleus. Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity. Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance

size1 :

0.05 mL

price1 :

235 USD

size2 :

0.1 mL

price2 :

350

size3 :

0.2 mL

price3 :

525

more info or order :

MyBioSource product webpage