Histone H3K27me3 Polyclonal Antibody | MyBioSource MBS126239 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Histone H3K27me3 Polyclonal Antibody

catalog :

MBS126239

quantity :

0.05 mL

price :

235 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

image 2 :

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody.

image 3 :

Immunohistochemistry of paraffin-embedded rat ovary using TryMethyl-Histone H3-K27 antibody MBS126239 at dilution of 1:100 (40x lens).

product information

catalog number :

MBS126239

products type :

Antibody

products full name :

Histone H3K27me3 Polyclonal Antibody

products short name :

[H3K27me3]

products name syn :

[TriMethyl-Histone H3-K27: HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; Histone H3/k; Histone H3/l; HIST3H3]

other names :

[Histone H3.1t; Histone H3.1t; histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T; histone cluster 3, H3; H3/g]

products gene name :

[H3K27me3]

other gene names :

[HIST3H3; HIST3H3; H3t; H3.4; H3/g; H3FT; H3FT; H3/t; H3t]

uniprot entry name :

H31T_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

rabbit

reactivity :

Human, Mouse, Rat, Other (Wide Range)

sequence length :

136

purity :

Affinity Purification

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP, CHIPseq

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200. IP: 1:50 - 1:200. ChIP: 1:20 - 1:100

image1 heading :

Western Blot (WB)

image2 heading :

Dot Blot (DB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Immunohistochemistry of paraffin-embedded human breast using TryMethyl-Histone H3-K27 antibody MBS126239 at dilution of 1:100 (40x lens)

image5 heading :

Immunohistochemistry (IHC)

image5 description :

Immunohistochemistry of paraffin-embedded human breast cancer using TryMethyl-Histone H3-K27 antibody MBS126239 at dilution of 1:100 (40x lens)

other info1 :

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

other info2 :

Immunogen: A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3. Calculated Molecular Weight: 15kDa

products categories :

Methylation

products description :

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

ncbi gi num :

18202512

ncbi acc num :

Q16695.3

uniprot acc num :

Q16695

ncbi pathways :

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chromosome Maintenance Pathway (161048); Condensation Of Prophase Chromosomes Pathway (1127547); DNA Damage/Telomere Stress Induced Senescence Pathway (905994); Disease Pathway (530764)

ncbi summary :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

uniprot summary :

HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 1q42. Cellular Component: nucleoplasm; nucleus. Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity. Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance

size1 :

0.05 mL

price1 :

235 USD

size2 :

0.1 mL

price2 :

350

size3 :

0.2 mL

price3 :

525

more info or order :

MyBioSource product webpage