PHB Polyclonal Antibody | MyBioSource MBS125679 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PHB Polyclonal Antibody

catalog :

MBS125679

quantity :

0.05 mL

price :

230 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS125679

products type :

Antibody

products full name :

PHB Polyclonal Antibody

products short name :

PHB

products name syn :

PHB; PHB1

other names :

Prohibitin; Prohibitin; prohibitin; epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; prohibitin

products gene name :

PHB

other gene names :

PHB; PHB; PHB1; HEL-215; HEL-S-54e

uniprot entry name :

PHB_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

272

purity :

Affinity Purification

concentration :

1mg/ml

storage stability :

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200

other info1 :

Species: Human. Immunogen: Recombinant Protein

other info2 :

Immunogen: Recombinant protein of human PHB. Calculated Molecular Weight: 30kDa

products categories :

Polyclonal

products description :

The prohibitins, called PHB1 and PHB2, are highly conserved proteins that are present in multiple compartments in eukaryotic cells. PHB1 is 30kDa tumor suppressor protein involved in cell cycle control (1). PHB1 has been found in mitochondria, the nucleus and the plasma membrane, as well as extracellularly in circulation (2). In mitochondria prohibitins mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (3,4). In the nucleus prohibitins interact with transcription factors such as Rb and p53 to regulate target gene transcription (2,5). Extracellular prohibitin can bind and activate C3 to enhance complement activation (6).

ncbi gi num :

464371

ncbi acc num :

P35232.1

uniprot acc num :

P35232

ncbi mol weight :

272

ncbi pathways :

Integrated Breast Cancer Pathway (219801)

ncbi summary :

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

uniprot summary :

PHB: a pleiotropic membrane protein that suppresses cell proliferation, apoptosis and senescence. It plays a role both in maintaining mitochondrial integrity and in cell cycle regulation. Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. It helps prevent ROS-induced senescence and its expression decreases heterogeneously during cellular aging. Helps maintain the angiogenic capacity of endothelial cells. Up-regulated during activation of primary human T cells via CD3/CD28 pathways. Present in multiple cellular compartments. Large assemblies of PHB and PHB2 localize in the inner membrane of mitochondria. Acts as a mitochondrial chaperone protein, regulates mitochondrial morphogenesis, and helps regulate the organization of mitochondrial DNA. Reported to target lipid rafts. In the nucleus, it has been reported to interact with various transcription factors, modulating their activity. A potential tumor suppressor that functions as a potent transcriptional corepressor for estrogen receptor alpha. It is downregulated by androgens, and represses androgen receptor activity. Co-localizes with Rb in the nucleus and recruits N-CoR and HDAC1 for transcriptional repression. In vivo promoter occupancy studies have suggested that the PHB gene is a direct target of c-Myc. May bind to and enhance the transcriptional activity of p53. Both Phb and Phb2 are present in the circulation and can be internalized by cultured cells. Overexpressed in endometrioid ovarian adenocarcinoma and papillary serous ovarian carcinoma. Mutated in sporadic breast cancer. Protein type: Oncoprotein; Cell cycle regulation; Mitochondrial; Chaperone; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Apoptosis. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: nucleoplasm; membrane; mitochondrion; integral to plasma membrane; mitochondrial inner membrane; cytoplasm; nucleus. Molecular Function: protein binding; enzyme binding; histone deacetylase binding. Biological Process: osteoblast differentiation; regulation of apoptosis; transcription from RNA polymerase II promoter; negative regulation of cell proliferation; progesterone receptor signaling pathway; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone deacetylation; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of transcription, DNA-dependent. Disease: Breast Cancer

size1 :

0.05 mL

price1 :

230 USD

size2 :

0.1 mL

price2 :

305

size3 :

0.2 mL

price3 :

460

more info or order :

MyBioSource product webpage