antibody

ZO1 Polyclonal Antibody

MBS125603

0.05 mg

285 USD

polyclonal

rabbit

nonconjugated

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

Antibody

ZO1 Polyclonal Antibody

ZO1

TJP1; DKFZp686M05161; MGC133289; ZO-1

Tight junction protein ZO-1; Tight junction protein ZO-1; tight junction protein ZO-1; zona occludens 1; zonula occludens 1 protein; tight junction protein 1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1

TJP1

TJP1; TJP1; ZO-1; ZO1

ZO1_HUMAN

Polyclonal

IgG

Rabbit

Human

1748

Affinity Purification

1mg/ml

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

WB: 1:500 - 1:1000. IHC: 1:50 - 1:100. IF: 1:50 - 1:100. FC: 1:20 - 1:50

Species: Human. Route: Recombinant Protein

Immunogen: Fusion protein of human ZO1. Calculated Molecular Weight: 195kDa

Polyclonal

Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 2). ZO-1 and -2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8).

85700443

Q07157.3

Q07157

1748

Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Apoptosis Pathway 105648!!Apoptotic Cleavage Of Cell Adhesion Proteins Pathway 105680!!Apoptotic Cleavage Of Cellular Proteins Pathway 105678!!Apoptotic Execution Phase Pathway 105677!!E-cadherin Signaling In The Nascent Adherens Junction Pathway 138005!!Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway 83102!!Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway 515!!Gap Junction Pathway 83072

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014]

0.05 mg

285 USD