HDAC4 Polyclonal Antibody | MyBioSource MBS125481 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

HDAC4 Polyclonal Antibody

catalog :

MBS125481

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using HDAC4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

image 2 :

Immunofluorescence analysis of A549 cells using HDAC4 antibody. Blue: DAPI for nuclear staining.

product information

catalog number :

MBS125481

products type :

Antibody

products full name :

HDAC4 Polyclonal Antibody

products short name :

[HDAC4]

products name syn :

[HDAC4; HA6116; HD4; HDAC-A; HDACA; KIAA0288]

other names :

[Histone deacetylase 4; Histone deacetylase 4; histone deacetylase 4; histone deacetylase A; histone deacetylase 4]

products gene name :

[HDAC4]

other gene names :

[HDAC4; HDAC4; HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A; KIAA0288; HD4]

uniprot entry name :

HDAC4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

1084

purity :

Affinity Purification

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF)

app notes :

WB: 1:500 - 1:2000. IF: 1:50 - 1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

other info2 :

Immunogen: A synthetic peptide of human HDAC4

products categories :

Polyclonal

products description :

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

ncbi gi num :

259016348

ncbi acc num :

P56524.3

uniprot acc num :

P56524

ncbi mol weight :

Calculated MW: 106kDa/119kDa. Observed MW: 117kDa

ncbi pathways :

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Cell Cycle Pathway (198811); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (771595); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (771593); Disease Pathway (530764); Endochondral Ossification Pathway (198812); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); FBXW7 Mutants And NOTCH1 In Cancer Pathway (771596)

ncbi summary :

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

uniprot summary :

HDAC4: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. Protein type: Hydrolase; EC 3.5.1.98; Deacetylase; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 2q37.3. Cellular Component: nucleoplasm; transcriptional repressor complex; histone deacetylase complex; cytoplasm; actomyosin; neuromuscular junction; nucleus; Z disc; cytosol; A band. Molecular Function: potassium ion binding; transcription activator binding; zinc ion binding; histone deacetylase binding; protein deacetylase activity; protein kinase binding; transcription factor binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H3-K14 specific); sequence-specific DNA binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity. Biological Process: cardiac muscle hypertrophy; response to drug; nervous system development; regulation of skeletal muscle fiber development; transcription, DNA-dependent; B cell activation; negative regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; histone deacetylation; response to denervation involved in regulation of muscle adaptation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; osteoblast development; positive regulation of protein sumoylation; chromatin remodeling; negative regulation of cell proliferation; B cell differentiation; regulation of protein binding; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of osteoblast differentiation; negative regulation of transcription, DNA-dependent; negative regulation of glycolysis; inflammatory response; skeletal development

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage