Phospho-RAC1-S71 Polyclonal Antibody | MyBioSource MBS125366 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Phospho-RAC1-S71 Polyclonal Antibody

catalog :

MBS125366

quantity :

0.05 mL

price :

235 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of A-431 cells, using Phospho-RAC1-S71 antibody at 1:1000 dilution. A431 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

product information

catalog number :

MBS125366

products type :

Antibody

products full name :

Phospho-RAC1-S71 Polyclonal Antibody

products short name :

[RAC1-S71]

products name syn :

[Phospho-RAC1-S71: MIG5; Rac-1; TC-25; p21-Rac1]

other names :

[Ras-related C3 botulinum toxin substrate 1; Ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1; ras-like protein TC25; cell migration-inducing gene 5 protein; ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1); Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1]

products gene name :

[RAC1]

other gene names :

[RAC1; RAC1; MIG5; Rac-1; TC-25; p21-Rac1; TC25]

uniprot entry name :

RAC1_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

rabbit

reactivity :

Human, Rat

sequence length :

192

purity :

Affinity Purification

concentration :

1mg/ml

storage stability :

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

tested application :

Western Blot (WB)

app notes :

WB: 1:500 - 1:2000

image1 heading :

Western Blot (WB)

other info1 :

Species: Human. Route: Synthetic Peptide

other info2 :

Immunogen: A phospho specific peptide corresponding to residues surrounding S71 of human RAC1. Calculated Molecular Weight: 21kDa

products categories :

Phosphorylation

products description :

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

ncbi gi num :

51702787

ncbi acc num :

P63000.1

uniprot acc num :

P63000

ncbi mol weight :

192

ncbi pathways :

AGE/RAGE Pathway (698754); Activation Of Rac Pathway (119530); Adaptive Immune System Pathway (366160); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Alpha6-Beta4 Integrin Signaling Pathway (198807); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Androgen Receptor Signaling Pathway (198806)

ncbi summary :

uniprot summary :

RAC1: a plasma membrane-associated member of the Rho-GTPase family. Plays a key role in cytoskeletal reorganization, membrane trafficking, transcriptional regulation and cell growth and development. GTP binding stimulates its activity. Phosphorylation by Akt may inhibit GTP binding of Rac1, therefore attenuating the downstream signal transduction pathway. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Two alternatively spliced isoforms have been described. Protein type: Motility/polarity/chemotaxis; G protein; G protein, monomeric, Rho; G protein, monomeric. Chromosomal Location of Human Ortholog: 7p22. Cellular Component: extrinsic to plasma membrane; Golgi membrane; focal adhesion; membrane; lamellipodium; cytoplasm; plasma membrane; melanosome; actin filament; trans-Golgi network; cytosol; phagocytic cup. Molecular Function: GTPase activity; protein binding; enzyme binding; GTP binding; GTP-dependent protein binding; Rho GDP-dissociation inhibitor binding; thioesterase binding; protein kinase binding; Rab GTPase binding. Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; metabolic process; positive regulation of apoptosis; cell motility involved in cell locomotion; regulation of cell migration; small GTPase mediated signal transduction; positive regulation of stress fiber formation; cell adhesion; bone resorption; platelet activation; anatomical structure morphogenesis; mast cell chemotaxis; dendrite morphogenesis; positive regulation of Rho protein signal transduction; regulation of defense response to virus by virus; negative regulation of interleukin-23 production; T cell costimulation; cerebral cortex radially oriented cell migration; actin cytoskeleton organization and biogenesis; axon guidance; cell-matrix adhesion; localization within membrane; positive regulation of focal adhesion formation; actin filament polymerization; response to wounding; ephrin receptor signaling pathway; inflammatory response; regulation of hydrogen peroxide metabolic process; lamellipodium biogenesis; embryonic olfactory bulb interneuron precursor migration; intercellular junction assembly and maintenance; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of phosphoinositide 3-kinase activity; engulfment of apoptotic cell; cell proliferation; G-protein coupled receptor protein signaling pathway; hyperosmotic response; positive regulation of actin filament polymerization; organization of an anatomical structure; auditory receptor cell morphogenesis; ruffle organization and biogenesis; innate immune response; negative regulation of receptor-mediated endocytosis; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; blood coagulation; cell motility; positive regulation of DNA replication

size1 :

0.05 mL

price1 :

235 USD

size2 :

0.1 mL

price2 :

350

size3 :

0.2 mL

price3 :

525

more info or order :

MyBioSource product webpage