beta-actin Polyclonal Antibody | MyBioSource MBS125142 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

beta-actin Polyclonal Antibody

catalog :

MBS125142

quantity :

0.05 mL

price :

160 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot analysis of extracts of various cells line, using B-actin antibody MBS125142 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:1000 dilution. Lysates/proteins: 25 ug/per lane. Blocking buffer: 3% non fat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.

image 2 :

Immunofluorescence analysis of HeLa cells using ACTB antibody (MBS125142) at dilution of 1:100 (40x lens). Blue: Dapi for nuclear staining.

product information

catalog number :

MBS125142

products type :

Antibody

products full name :

beta-actin Polyclonal Antibody

products short name :

[beta-actin]

products name syn :

[BRWS1;PS1TP5BP1]

other names :

[PS1TP5-binding protein 1; HCG15971, isoform CRA_a; actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1; actin, beta; HCG15971, isoform CRA_a]

products gene name :

[beta-actin]

products gene name syn :

[b-actin]

other gene names :

[ACTB; PS1TP5BP1; BRWS1; PS1TP5BP1]

uniprot entry name :

Q1KLZ0_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

375

purity :

Affinity Purification

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

WB, IHC, IF

app notes :

WB 1:500 -1:2000. IHC 1:50 -1:200. IF 1:50 -1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Calculated MW: 41 kDa. Observed MW: 42kDa

other info2 :

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human ACTB (NP_001092.1).

products categories :

Control pAb

products description :

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

ncbi gi num :

121940727

ncbi acc num :

Q1KLZ0

uniprot acc num :

Q1KLZ0

ncbi pathways :

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Axon Guidance Pathway (105688); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Chaperonin-mediated Protein Folding Pathway (106242); Chromatin Modifying Enzymes Pathway (1127682)

ncbi summary :

uniprot summary :

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 7p22. Cellular Component: cortical cytoskeleton; extracellular space; NuA4 histone acetyltransferase complex; focal adhesion; protein complex; postsynaptic density; cytosol; ribonucleoprotein complex; nucleoplasm; cytoskeleton; membrane; axon; cytoplasm; nuclear chromatin; plasma membrane. Molecular Function: protein binding; nucleosomal DNA binding; structural constituent of cytoskeleton; kinesin binding; Tat protein binding; nitric-oxide synthase binding; protein kinase binding; ATP binding. Biological Process: axon guidance; intercellular junction assembly and maintenance; establishment and/or maintenance of chromatin architecture; protein folding; de novo posttranslational protein folding; retinal homeostasis; substantia nigra development; cellular protein metabolic process; ephrin receptor signaling pathway; ATP-dependent chromatin remodeling; innate immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation; cell motility. Disease: Dystonia, Juvenile-onset; Baraitser-winter Syndrome 1

size1 :

0.05 mL

price1 :

160 USD

size2 :

0.1 mL

price2 :

200

size3 :

0.2 mL

price3 :

275

more info or order :

MyBioSource product webpage