NGF Polyclonal Antibody | MyBioSource MBS125020 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

NGF Polyclonal Antibody

catalog :

MBS125020

quantity :

0.05 mL

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts of various cell lines, using NGF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

product information

catalog number :

MBS125020

products type :

Antibody

products full name :

NGF Polyclonal Antibody

products short name :

[NGF]

products name syn :

[NGF; Beta-NGF; HSAN5; NGFB; nerve growth factor]

other names :

[Beta-nerve growth factor; Beta-nerve growth factor; beta-nerve growth factor; nerve growth factor, beta subunit; nerve growth factor (beta polypeptide)]

products gene name :

[NGF]

other gene names :

[NGF; NGF; NGFB; HSAN5; Beta-NGF; NGFB; Beta-NGF]

uniprot entry name :

NGF_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

241

purity :

Affinity Purification

form :

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

storage stability :

Store at -20°C. Avoid freeze / thaw cycles.

tested application :

Western Blot (WB)

app notes :

WB: 1:500 - 1:2000

image1 heading :

Western Blot (WB)

other info2 :

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-241 of human NGF (NP_002497.2). Calculated MW: 26 kDa. Observed MW: 17 kDa, 27 kDa.

products categories :

Polyclonal

products description :

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.

ncbi gi num :

90110037

ncbi acc num :

P01138.3

uniprot acc num :

P01138

ncbi mol weight :

Calculate MW: 26 kDa. Observed MW: 17kDa/27kDa

ncbi pathways :

ARMS-mediated Activation Pathway (106466); Activation Of TRKA Receptors Pathway (106456); Apoptosis Pathway (83060); Apoptosis Pathway (470); Axonal Growth Stimulation Pathway (106453); BDNF Signaling Pathway (712093); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (106443); Ceramide Signalling Pathway (106451); Frs2-mediated Activation Pathway (106465); Id Signaling Pathway (198871)

ncbi summary :

uniprot summary :

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p13.1. Cellular Component: extracellular space; Golgi lumen; cytoplasmic membrane-bound vesicle; extracellular region; endosome. Molecular Function: metalloendopeptidase inhibitor activity; protein binding; nerve growth factor receptor binding; growth factor activity; receptor signaling protein activity. Biological Process: response to nicotine; circadian rhythm; response to peptide hormone stimulus; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of apoptosis; adult locomotory behavior; regulation of neuron differentiation; positive regulation of axon extension; response to glucocorticoid stimulus; regulation of neurotransmitter secretion; response to lipopolysaccharide; regulation of caspase activity; sensory perception of pain; regulation of axonogenesis; negative regulation of cell cycle; nerve growth factor processing; response to radiation; cell-cell signaling; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; response to electrical stimulus; inflammatory response; response to drug; positive regulation of neuron maturation; phosphoinositide-mediated signaling; positive regulation of protein amino acid autophosphorylation; positive regulation of nerve growth factor receptor signaling pathway; regulation of release of sequestered calcium ion into cytosol; memory; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; response to mechanical stimulus; phospholipase C activation; response to ozone; Ras protein signal transduction; positive regulation of axonogenesis; positive regulation of transcription factor activity; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis. Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V

size1 :

0.05 mL

price1 :

200 USD

size2 :

0.1 mL

price2 :

275

size3 :

0.2 mL

price3 :

430

more info or order :

MyBioSource product webpage