antibody

DDDDK-Tag Monoclonal Antibody

MBS125006

0.05 mg

200 USD

monoclonal

mouse

nonconjugated

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Antibody

DDDDK-Tag Monoclonal Antibody

DDDDK-Tag

Mouse anti-DDDDK mAb-BET: N/A

DDDDK-Tag

Monoclonal

IgG

Mouse

Affinity Purification

1mg/ml

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

WB: 1:2000 - 1:5000. IHC: 1:50 - 1:500. IF: 1:50 - 1:500. IP: 1:50 - 1:100

Immunogen: Fusion protein of human DDDDK-tag

Tag mAb

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins.

0.05 mg

200 USD