beta-actin Monoclonal Antibody | MyBioSource MBS125003 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

beta-actin Monoclonal Antibody

catalog :

MBS125003

quantity :

0.05 mL

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS125003

products type :

Antibody

products full name :

beta-actin Monoclonal Antibody

products short name :

beta-actin

products name syn :

Mouse anti beta-actin mAb: Mouse anti b-actin mAb; ACTB; PS1TP5BP1; beta-actin; beta-actin

other names :

PS1TP5-binding protein 1; HCG15971, isoform CRA_a; actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1; actin, beta; HCG15971, isoform CRA_a

products gene name :

beta-actin

products gene name syn :

b-actin

other gene names :

ACTB; PS1TP5BP1; BRWS1; PS1TP5BP1

uniprot entry name :

Q1KLZ0_HUMAN

clonality :

Monoclonal

isotype :

IgG

host :

Mouse

reactivity :

Human, Mouse, Rat

sequence length :

375

purity :

Affinity Purification

concentration :

1mg/ml

storage stability :

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

tested application :

Western Blot (WB)

app notes :

WB: 1:2000 - 1:5000

other info2 :

Immunogen: Fusion protein of human beta-actin. Calculated Molecular Weight: 42kDa

products categories :

Control mAb

products description :

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta Actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody was generated by full length beta actin protein and can cross-react with other actins.

ncbi gi num :

121940727

ncbi acc num :

Q1KLZ0

uniprot acc num :

Q1KLZ0

ncbi mol weight :

41,737 Da

ncbi pathways :

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Axon Guidance Pathway (105688); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Chaperonin-mediated Protein Folding Pathway (106242); Chromatin Modifying Enzymes Pathway (1127682)

ncbi summary :

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

uniprot summary :

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 7p22. Cellular Component: cortical cytoskeleton; extracellular space; NuA4 histone acetyltransferase complex; protein complex; focal adhesion; postsynaptic density; cytosol; ribonucleoprotein complex; nucleoplasm; cytoskeleton; membrane; axon; cytoplasm; nuclear chromatin; plasma membrane. Molecular Function: protein binding; nucleosomal DNA binding; structural constituent of cytoskeleton; kinesin binding; Tat protein binding; nitric-oxide synthase binding; protein kinase binding; ATP binding. Biological Process: axon guidance; intercellular junction assembly and maintenance; establishment and/or maintenance of chromatin architecture; protein folding; de novo posttranslational protein folding; retinal homeostasis; substantia nigra development; cellular protein metabolic process; ephrin receptor signaling pathway; ATP-dependent chromatin remodeling; innate immune response; cell motility; vascular endothelial growth factor receptor signaling pathway; blood coagulation. Disease: Dystonia, Juvenile-onset; Baraitser-winter Syndrome 1

size1 :

0.05 mL

price1 :

190 USD

size2 :

0.1 mL

price2 :

230

size3 :

0.2 mL

price3 :

305

more info or order :

MyBioSource product webpage