catalog number :
MBS1248736
products type :
Recombinant Protein
products full name :
Recombinant Escherichia coli Polyphosphate kinase (ppk)
products short name :
[Polyphosphate kinase (ppk)]
other names :
[polyphosphate kinase, component of RNA degradosome; Polyphosphate kinase; polyphosphate kinase, component of RNA degradosome; ATP-polyphosphate phosphotransferase]
products gene name :
[ppk]
other gene names :
[ppk; ppk; ECK2497; JW2486]
host :
E Coli or Yeast or Baculovirus or Mammalian Cell
sequence positions :
[2-688. Full Length of Mature Protein]
sequence :
GQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLG
IYSNNLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGK
IQSRVLKADQEFDGLYNELLLEMARNQIFLINERQLSVN
QQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYL
AVEIIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPM
ILLDNILRYCLDDIFKGFFDYDALNAYSMKMTRDAEYDL
VHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVE
VLREKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVN
KPLPRLRHIWFDKAQFRNGFDAIRERDVLLYYPYHTFEH
VLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAH
NGKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAP
GLKIHAKLFLISRKENGEVVRYAHIGTGNFNEKTARLYT
DYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVD
RLYAASSSGVPVNLLVRGMCSLIPNLEGISDNIRAISIV
DRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEVA
TPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVP
RGNRRKVRAQLAIYDYIKSLEQPE
form :
Liquid containing glycerol
storage stability :
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
other info1 :
Species: Escherichia coli (strain K12)
other info2 :
Storage Buffer: Tris-based buffer, 50% glycerol. Production Note: Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to support@mybiosource.com for more details.
ncbi acc num :
NP_416996.1
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
80,432 Da
ncbi pathways :
Oxidative Phosphorylation Pathway (1020); Oxidative Phosphorylation Pathway (303); RNA Degradation Pathway (116132); RNA Degradation Pathway (116127)
ncbi summary :
The role of polyphosphate (poly(Pi)) in Escherichia coli is still not fully understood; it may function in energy storage. [More information is available at EcoCyc: EG11510].
uniprot summary :
Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). Can form linear polymers of orthophosphate with chain lengths up to 1000 or more. Can use GTP instead of ATP, but the efficiency of GTP is 5% that of ATP. Also exhibits several other enzymatic activities, which include: ATP synthesis from polyP in the presence of excess ADP, general nucleoside-diphosphate kinase activity, linear guanosine 5'-tetraphosphate (ppppG) synthesis and autophosphorylation.
size7 :
0.05 mg (Baculovirus)
size9 :
0.05 mg (Mammalian-Cell)
size12 :
0.1 mg (Mammalian-Cell)
size13 :
0.1 mg (Baculovirus)
size14 :
0.5 mg (Baculovirus)
size16 :
1 mg (Baculovirus)