Recombinant Human Elongation factor 2 | MyBioSource MBS1213669 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Elongation factor 2

catalog :

MBS1213669

quantity :

0.05 mg (E-Coli)

price :

180 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS1213669

products type :

Recombinant Protein

products full name :

Recombinant Human Elongation factor 2

products short name :

Elongation factor 2

other names :

elongation factor 2; Elongation factor 2; elongation factor 2; eukaryotic translation elongation factor 2

products gene name :

EEF2

products gene name syn :

EF2

other gene names :

EEF2; EEF2; EF2; EF-2; EEF-2; SCA26; EF2; EF-2

uniprot entry name :

EF2_HUMAN

host :

E Coli or Yeast or Baculovirus or Mammalian Cell

sequence positions :

2-858, Full length

sequence length :

858

sequence :

VNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLV
CKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFY
ELSENDLNFIKQSKDGAGFLINLIDSPGHVDFSSEVTAA
LRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMM
NKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGE
SGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAK
FAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFS
KSATSPEGKKLPRTFCQLILDPIFKVFDAIMNFKKEETA
KLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQ
MITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPK
GPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIM
GPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNI
VGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVA
VEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIA
GAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEE
SNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSAR
QELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTD
ITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFD
VHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLME
PIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPM
FVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQIL
PGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL

purity :

Greater than 90% as determined by SDS-PAGE.

form :

Liquid containing glycerol; lyophilization may be available upon request.

storage stability :

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

products categories :

Epigenetics and Nuclear Signaling

products description :

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movent of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

products references :

Complete sequence of the coding region of human elongation factor 2 (EF-2) by enzymatic amplification of cDNA from human ovarian granulosa cells.Rapp G., Klaudiny J., Hagendorff G., Luck M.R., Heinz K.Biol. Chem. Hoppe-Seyler 370:1071-1075(1989) Construction of a plasmid containing the complete coding region of human elongation factor 2.Hanes J., Freudenstein J., Rapp G., Scheit K.H.Biol. Chem. Hoppe-Seyler 373:201-204(1992) Ustek D., Bektas M., Cakiris A., Oku B., Bermek E.

ncbi gi num :

4503483

ncbi acc num :

NP_001952.1

ncbi gb acc num :

NM_001961.3

uniprot acc num :

P13639

ncbi mol weight :

99.2kD

ncbi pathways :

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); BDNF Signaling Pathway (712093); Disease Pathway (1268854); Eukaryotic Translation Elongation Pathway (1268690); Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway (1268702); Gene Expression Pathway (1269649); Infectious Disease Pathway (1269056); Metabolism Of Proteins Pathway (1268677)

ncbi summary :

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]

uniprot summary :

EEF2: a member of the GTP-binding translation elongation factor family. An essential factor for protein synthesis. Promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by eEF2 kinase phosphorylation. eEF2 kinase is normally dependent on Ca2+ ions and calmodulin. eEF2 kinase can also be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. Protein type: Translation; Translation elongation. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: cytoplasm; cytosol; lipid raft; membrane; nucleus; plasma membrane; polysomal ribosome; ribonucleoprotein complex. Molecular Function: 5S rRNA binding; actin filament binding; GTP binding; GTPase activity; p53 binding; protein binding; protein kinase binding; ribosome binding; translation elongation factor activity. Biological Process: aging; cellular protein metabolic process; gene expression; glial cell proliferation; hemopoietic progenitor cell differentiation; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; positive regulation of translation; post-translational protein modification; response to estradiol stimulus; response to ethanol; response to fluoxetine; response to folic acid; response to hydrogen peroxide; skeletal muscle contraction; translation; translational elongation. Disease: Spinocerebellar Ataxia 26

size1 :

0.05 mg (E-Coli)

price1 :

180 USD

size2 :

0.2 mg (E-Coli)

price2 :

490

size3 :

0.5 mg (E-Coli)

price3 :

715

size4 :

0.05 mg (Baculovirus)

price4 :

950

size5 :

1 mg (E-Coli)

price5 :

1170

more info or order :

MyBioSource product webpage