Mouse monoclonal antibody Anti-Human TFEB | MyBioSource MBS120432 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse monoclonal antibody Anti-Human TFEB

catalog :

MBS120432

quantity :

0.1 mg

price :

345 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

319C2a

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, dot blot

more info or order :

MyBioSource product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; loading ...; fig s2c	Bartolomeo R, Cinque L, De Leonibus C, Forrester A, Salzano A, Monfregola J, et al. mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J Clin Invest. 2017;127:3717-3729 pubmed publisher
immunohistochemistry; human; loading ...; fig 8	Nascimbeni A, Fanin M, Angelini C, Sandri M. Autophagy dysregulation in Danon disease. Cell Death Dis. 2017;8:e2565 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig s1	Angelini C, Nascimbeni A, Cenacchi G, Tasca E. Lipolysis and lipophagy in lipid storage myopathies. Biochim Biophys Acta. 2016;1862:1367-73 pubmed publisher
immunocytochemistry; mouse; 1:50	Settembre C, Medina D. TFEB and the CLEAR network. Methods Cell Biol. 2015;126:45-62 pubmed publisher
immunocytochemistry; rat; fig 2b western blot; rat; fig 2 western blot; mouse; fig 4a	Chua J, Reddy S, Merry D, Adachi H, Katsuno M, Sobue G, et al. Transcriptional activation of TFEB/ZKSCAN3 target genes underlies enhanced autophagy in spinobulbar muscular atrophy. Hum Mol Genet. 2014;23:1376-86 pubmed publisher
	Bento C, Ashkenazi A, Jimenez Sanchez M, Rubinsztein D. The Parkinson's disease-associated genes ATP13A2 and SYT11 regulate autophagy via a common pathway. Nat Commun. 2016;7:11803 pubmed publisher
	Settembre C, Zoncu R, Medina D, Vetrini F, Erdin S, Erdin S, et al. A lysosome-to-nucleus signalling mechanism senses and regulates the lysosome via mTOR and TFEB. EMBO J. 2012;31:1095-108 pubmed publisher

product information

catalog number :

MBS120432

products type :

Antibody

products full name :

Mouse monoclonal antibody Anti-Human TFEB

products short name :

TFEB

products name syn :

Homo sapiens transcription factor EB (TFEB), mRNA.; TCFEB; AlphaT

other names :

Homo sapiens transcription factor EB (TFEB), transcript variant 1, mRNA; Transcription factor EB; transcription factor EB; T-cell transcription factor EB; class E basic helix-loop-helix protein 35; transcription factor EB; Class E basic helix-loop-helix protein 35; bHLHe35

products gene name :

TFEB

other gene names :

TFEB; TFEB; TCFEB; BHLHE35; ALPHATFEB

uniprot entry name :

TFEB_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

319C2a

host :

Mouse

reactivity :

Human

sequence length :

2364

form :

Mouse monoclonal anti-human TFEB antibody in PBS (3.0 mM KCl, 1.5 mM KH 4 PO 4 . 140mM NaCl, 8.0mM Na 2 HPO 4 (pH7.4) Containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN 3 ).

concentration :

100ug/ml (1.0 ml)

storage stability :

Store at 2-8 degree C for up to one year. We recommend storing at -20 degree C for long term storage. Avoid repeat freezing and thawing cycles.

tested application :

Dot Blot (DB) . ICC (immunocytochemistry). Western Blot (WB)

app notes :

ICC:2-100ug/ml

other info1 :

Preparation: This antibody was purified using protein A column chromatography from ascites. Immunogen: Recombinant

other info2 :

Sterility: Filtered through a 0.22 um membrane. Safety warnings and precautions: Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at one with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laborartory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off. Disposal: This antibody solution contains sodium azide (NaN 3 ) as a preservative. There is a potential hazard that NaN 3 reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.

products description :

Probable transcription factor binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer.[UniProtKB Function]

ncbi gi num :

268607698

ncbi acc num :

NP_009093.1

ncbi gb acc num :

NM_007162

uniprot summary :

TFEB: Transcription factor that specifically recognizes and binds E-box sequences (3 -CANNTG-5 ). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5 -GTCACGTGAC-3 ) present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Homodimer and heterodimer; with TFE3 or MITF. Belongs to the MiT/TFE family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 6p21. Cellular Component: cytoplasm; nucleus. Molecular Function: protein dimerization activity; transcription factor activity. Biological Process: embryonic placenta development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; lysosome organization and biogenesis; autophagy; positive regulation of transcription from RNA polymerase II promoter; positive regulation of autophagy; humoral immune response

size1 :

0.1 mg

price1 :

345 USD

more info or order :

MyBioSource product webpage