antibody

Mouse monoclonal antibody Anti-Human NFS1

MBS120342

0.1 mg

345 USD

monoclonal

mouse

nonconjugated

2635E1a

Antibody

Mouse monoclonal antibody Anti-Human NFS1

NFS1

Homo sapiens NFS1 nitrogen fixation 1 homolog (S. cerevisiae); IscS; NIFS; HUSSY-08

Homo sapiens NFS1 cysteine desulfurase (NFS1), transcript variant 1, mRNA; Cysteine desulfurase, mitochondrial; cysteine desulfurase, mitochondrial; NFS1 nitrogen fixation 1 homolog; nitrogen fixation 1 (S. cerevisiae, homolog); nitrogen-fixing bacteria S-like protein; NFS1 cysteine desulfurase

NFS1

NFS1; NFS1; IscS; NIFS; HUSSY-08

NFS1_HUMAN

Monoclonal

IgG1

2635E1a

Mouse

Human

2385

Mouse monoclonal anti-human NFS1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2PO4, 140mM NaCl, 8.0mM Na2HPO4(pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).

100 ug/ml (1.0 ml)

Store at 2-8 degree C for up to one year. We recommend storing at -20 degree C for long-term storage. Avoid repeat freezing and thawing cycles.

Dot Blot (DB), Western Blot (WB)

Recommended condition: WB: 0.2-2ug/ml

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Sterility: Filtered through a 0.22 um membrane. Disposal: This antibody solution contains sodium azide (NaN3) as a preservative. There is a potential hazard that NaN3 reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. Safety warning and precautions: Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off.

Iron-sulfur clusters are required for the function of many cellular enzymes. The protein encoded by this gene supplies inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded protein belongs to the class-V family of pyridoxal phosphate-dependent aminotransferases. [NCBI Entrez Gene Summary]

312222766

NP_066923.3

NM_021100

44,362 Da

Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (906010); Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway (906009); Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway (906005)

Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010]

NFS1: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron- sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily. 2 isoforms of the human protein are produced by alternative initiation. Protein type: Mitochondrial; Cofactor and Vitamin Metabolism - thiamine; Transferase; EC 2.8.1.7. Chromosomal Location of Human Ortholog: 20q11.22. Cellular Component: nucleoplasm; mitochondrion; intracellular membrane-bound organelle; mitochondrial matrix; cytoplasm; nucleolus; cytosol; nucleus. Molecular Function: protein binding; protein homodimerization activity; cysteine desulfurase activity; pyridoxal phosphate binding. Biological Process: cysteine metabolic process; iron incorporation into metallo-sulfur cluster; vitamin metabolic process; sulfur amino acid metabolic process; protein complex assembly; Mo-molybdopterin cofactor biosynthetic process; molybdopterin cofactor biosynthetic process; water-soluble vitamin metabolic process

0.1 mg

345 USD