Mouse monoclonal antibody Anti-Human EHMT1 | MyBioSource MBS120327 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse monoclonal antibody Anti-Human EHMT1

catalog :

MBS120327

quantity :

0.1 mg

price :

345 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2312C2a

reactivity :

human

application :

immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS120327

products type :

Antibody

products full name :

Mouse monoclonal antibody Anti-Human EHMT1

products short name :

EHMT1

products name syn :

Homosapienseuchromatichistone-lysineN-methyltransferase1; GLP;FP13812;FLJ12879;KIAA1876;EUHMTASE1;Eu-HMTase1;bA188C12.1;DKFZp667M072;RP11-188C12.1

other names :

Homo sapiens euchromatic histone-lysine N-methyltransferase 1 (EHMT1), transcript variant 1, mRNA; Histone-lysine N-methyltransferase EHMT1; histone-lysine N-methyltransferase EHMT1; G9a-like protein 1; H3-K9-HMTase 5; histone H3-K9 methyltransferase 5; histone-lysine N-methyltransferase, H3 lysine-9 specific 5; lysine N-methyltransferase 1D; euchromatic histone-lysine N-methyltransferase 1; Euchromatic histone-lysine N-methyltransferase 1; Eu-HMTase1; G9a-like protein 1; GLP; GLP1; Histone H3-K9 methyltransferase 5; H3-K9-HMTase 5; Lysine N-methyltransferase 1D

products gene name :

EHMT1

other gene names :

EHMT1; EHMT1; GLP; GLP1; KMT1D; FP13812; EUHMTASE1; Eu-HMTase1; bA188C12.1

uniprot entry name :

EHMT1_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

2312C2a

host :

Mouse

reactivity :

Human

sequence length :

5123

concentration :

100 ug/ml (1.0 ml)

tested application :

Dot Blot (DB), Immunocytochemistry (ICC)

other info1 :

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

other info2 :

Sterility: Filtered through a 0.22 um membrane.

products description :

Histone methyltransferase. Methylates 'Lys-9' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. [UniProtKB Function]

ncbi gi num :

224465232

ncbi acc num :

NP_079033.4

ncbi gb acc num :

NM_024757

ncbi mol weight :

86,704 Da

ncbi pathways :

Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chromatin Modifying Enzymes Pathway (1127682); Chromatin Organization Pathway (1127681); Lysine Degradation Pathway (82956); Lysine Degradation Pathway (320); PKMTs Methylate Histone Lysines Pathway (1127686); Senescence-Associated Secretory Phenotype (SASP) Pathway (905996)

ncbi summary :

The protein encoded by this gene is a histone methyltransferase that is part of the E2F6 complex, which represses transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome, also known as Kleefstra syndrome). Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

uniprot summary :

EHMT1: Histone methyltransferase that specifically mono- and dimethylates Lys-9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates Lys-27 of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non- histone proteins: mediates dimethylation of Lys-373 of p53/TP53. Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome). Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems. Belongs to the histone-lysine methyltransferase family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.1.1.43; Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; Methyltransferase. Chromosomal Location of Human Ortholog: 9q34.3. Cellular Component: nucleoplasm; cytoplasm; plasma membrane; chromosome; nucleus. Molecular Function: methyltransferase activity; protein binding; zinc ion binding; p53 binding; protein-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K9 specific); histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific). Biological Process: histone methylation; embryonic development; establishment and/or maintenance of chromatin architecture; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; negative regulation of transcription, DNA-dependent; histone H3-K9 methylation. Disease: Kleefstra Syndrome

size1 :

0.1 mg

price1 :

345 USD

more info or order :

MyBioSource product webpage