Mouse monoclonal antibody Anti-Human CBLB | MyBioSource MBS120301 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse monoclonal antibody Anti-Human CBLB

catalog :

MBS120301

quantity :

0.1 mg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

246C5a

reactivity :

human

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS120301

products type :

Antibody

products full name :

Mouse monoclonal antibody Anti-Human CBLB

products short name :

CBLB

products name syn :

Homo sapiens Cas-Br-M (murine) ecotropic retroviral transforming sequence b; RNF56; Nbla00127

other names :

Homo sapiens Cas-Br-M (murine) ecotropic retroviral transforming sequence b, mRNA; E3 ubiquitin-protein ligase CBL-B; E3 ubiquitin-protein ligase CBL-B; Cas-Br-M (murine) ecotropic retroviral transforming sequence b; Cas-Br-M (murine) ectropic retroviral transforming sequence b; Cbl proto-oncogene, E3 ubiquitin protein ligase B; RING finger protein 56; SH3-binding protein CBL-B; casitas B-lineage lymphoma proto-oncogene b; signal transduction protein CBL-B; Cbl proto-oncogene B, E3 ubiquitin protein ligase; Casitas B-lineage lymphoma proto-oncogene b; RING finger protein 56; SH3-binding protein CBL-B; Signal transduction protein CBL-B

products gene name :

CBLB

other gene names :

CBLB; CBLB; Cbl-b; RNF56; Nbla00127

uniprot entry name :

CBLB_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

246C5a

host :

Mouse

reactivity :

Human

sequence length :

3923

concentration :

100 ug/ml (1.0 ml)

tested application :

Dot Blot (DB), Western Blot (WB)

other info1 :

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

other info2 :

Sterility: Filtered through a 0.22 um membrane.

ncbi gi num :

34192827

ncbi acc num :

BC032851

ncbi mol weight :

86,582 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway (576249); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); B Cell Receptor Signaling Pathway (198909); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); CD40/CD40L Signaling Pathway (138061); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Chronic Myeloid Leukemia Pathway (83116); Chronic Myeloid Leukemia Pathway (528)

uniprot summary :

Cbl-b: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B- cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T- cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization. Interacts with SH3 domain-containing proteins LCK, CRK and SORBS1. Interacts with LCP2 and ZAP70. May interact with CBL. Interacts with SH3 domain-containing proteins VAV1, FYN, FGR, PLCG1, GRB2, CRKL, PIK3R1 and SH3KBP1/CIN85. Identified in heterotrimeric complexes with SH3KBP1/CIN85, CD2AP and ARHGEF7, where one CBLB peptide binds two copies of the other protein. Interacts with poly-ubiquitinated proteins. Dimerization is required for the binding of poly-ubiquitin, but not for the binding of mono-ubiquitin. Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle. Present in lymphocytes. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Ligase; Adaptor/scaffold; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system; Calcium-binding. Chromosomal Location of Human Ortholog: 3q13.11. Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol. Molecular Function: signal transducer activity; protein binding; phosphotyrosine binding; zinc ion binding; ubiquitin-protein ligase activity; calcium ion binding; ligase activity. Biological Process: cell surface receptor linked signal transduction; T cell activation; positive regulation of protein catabolic process; negative regulation of T cell receptor signaling pathway; protein ubiquitination; negative regulation of alpha-beta T cell proliferation; immune response; NLS-bearing substrate import into nucleus; signal transduction; positive regulation of T cell anergy

size1 :

0.1 mg

more info or order :

MyBioSource product webpage