antibody

Mouse monoclonal antibody Anti-Human DDX5

MBS120235

0.1 mg

345 USD

monoclonal

mouse

nonconjugated

2257C3a

western blot, immunocytochemistry, immunoprecipitation

Antibody

Mouse monoclonal antibody Anti-Human DDX5

DDX5

Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5); p68; HLR1; G17P1; HUMP68; DKFZp686J01190

Homo sapiens DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5), mRNA; Probable ATP-dependent RNA helicase DDX5; probable ATP-dependent RNA helicase DDX5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD box protein 5; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); RNA helicase p68; DEAD (Asp-Glu-Ala-Asp) box helicase 5; DEAD box protein 5; RNA helicase p68

DDX5

DDX5; DDX5; p68; HLR1; G17P1; HUMP68

DDX5_HUMAN

Monoclonal

IgG1

2257C3a

Mouse

Human

3769

Mouse monoclonal anti-human DDX5antibody in PBS (3.0 mM KCl, 1.5 mM KH PO , 140 mM NaCl, 8.0 mM Na HPO (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3)

100 ug/ml (1.0 ml)

Store at 2-8 degree C for up to one year. We recommend storing at –20 degree C for long-term storage. Avoid repeat freezing and thawing cycles

Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)

WB: 0.2-2ug/ml. IP: 100-500ug/sample. ICC: 2-100 ug/ml

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Immunogen: Recombinant

Sterility: Filtered through a 0.22 um membrane. Disposal: This antibody solution contains sodium azide (NaN )as a preservative. There is a potential hazard that NaN3 reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [NCBI Entrez Gene Summary]

221139768

NP_004387.1

NM_004396

60,563 Da

Direct P53 Effectors Pathway (137939); Proteoglycans In Cancer Pathway (782000); Proteoglycans In Cancer Pathway (782054); Spliceosome Pathway (125136); Spliceosome Pathway (124832); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq, Jul 2008]

DDX5: a putative ATP-dependent RNA helicase. A proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Belongs to the DEAD box helicase family. DDX5/DDX17 subfamily. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Protein type: Nucleolus; EC 3.6.4.13; RNA-binding; RNA splicing; Spliceosome; Nuclear receptor co-regulator; Helicase. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: nucleoplasm; membrane; nucleolus; nucleus; ribonucleoprotein complex. Molecular Function: calmodulin binding; protein binding; enzyme binding; androgen receptor binding; transcription coactivator activity; estrogen receptor binding; calcium-dependent protein binding; ATP-dependent RNA helicase activity; ATP binding; RNA helicase activity. Biological Process: nuclear mRNA splicing, via spliceosome; circadian rhythm; transcription, DNA-dependent; regulation of osteoblast differentiation; in utero embryonic development; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator; cell growth; regulation of viral genome replication; regulation of alternative nuclear mRNA splicing, via spliceosome

0.1 mg

345 USD