Mouse monoclonal antibody Anti-Human RUVBL1 | MyBioSource MBS120207 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse monoclonal antibody Anti-Human RUVBL1

catalog :

MBS120207

quantity :

0.1 mg

price :

345 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2943C1a

reactivity :

human

application :

western blot, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS120207

products type :

Antibody

products full name :

Mouse monoclonal antibody Anti-Human RUVBL1

products short name :

RUVBL1

products name syn :

Homo sapiens RuvB-like 1 (E. coli) (RUVBL1); RVB1; ECP54;TIP49; NMP238; TIP49A

other names :

Homo sapiens RuvB-like AAA ATPase 1 (RUVBL1), mRNA; RuvB-like 1; ruvB-like 1; 49 kDa TATA box-binding protein-interacting protein; 49 kDa TBP-interacting protein; 54 kDa erythrocyte cytosolic protein; ECP-54; INO80 complex subunit H; NMP 238; RuvB (E coli homolog)-like 1; TAP54-alpha; TATA binding protein interacting protein 49 kDa; TIP60-associated protein 54-alpha; nuclear matrix protein 238; pontin 52; RuvB-like AAA ATPase 1; 49 kDa TATA box-binding protein-interacting protein; 49 kDa TBP-interacting protein; 54 kDa erythrocyte cytosolic protein; ECP-54; INO80 complex subunit H; Nuclear matrix protein 238; NMP 238; Pontin 52; TIP49a; TIP60-associated protein 54-alpha; TAP54-alpha

products gene name :

RUVBL1

other gene names :

RUVBL1; RUVBL1; RVB1; TIH1; ECP54; TIP49; INO80H; NMP238; PONTIN; TIP49A; Pontin52

uniprot entry name :

RUVB1_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

2943C1a

host :

Mouse

reactivity :

Human

sequence length :

1785

concentration :

100 ug/ml (1.0 ml)

tested application :

Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)

other info1 :

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

other info2 :

Sterility: Filtered through a 0.22 um membrane.

products description :

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. [UniProtKB Function]

ncbi gi num :

197276633

ncbi acc num :

NP_003698.1

ncbi gb acc num :

NM_003707

ncbi mol weight :

42,127 Da

ncbi pathways :

C-MYC Pathway (169344); Cell Cycle Pathway (530733); Chromatin Modifying Enzymes Pathway (1127682); Chromatin Organization Pathway (1127681); Chromosome Maintenance Pathway (161048); Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway (161050); Disease Pathway (530764); Extension Of Telomeres Pathway (106541); Gastric Cancer Network 1 Pathway (760635); HATs Acetylate Histones Pathway (1127683)

uniprot summary :

RUVBL1: Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (3 to 5 ) activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1- CTNNB1 complex. Belongs to the ruvB family. Forms homotypic and heterotypic interactions. Forms a multimeric complex with RUVBL2. Interacts with the transcriptional activation domain of MYC. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL1 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Component of the BAF53 complex, at least composed of ACTL6A/BAF53A, RUVBL1/TIP49, SMARCA2/BRM, and TRRAP/PAF400. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Associates with alpha and gamma tubulins, particularly during metaphase and early anaphase. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex, at least composed of ACTL6A, ACTR5, ACTR8, RVBL1, RVBL2, INO80, INO80B, INO80C, INO80D and INO80E. Interacts with IGHMBP2. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; EC 3.6.4.12; Helicase. Chromosomal Location of Human Ortholog: 3q21. Cellular Component: nucleoplasm; Golgi apparatus; NuA4 histone acetyltransferase complex; nuclear matrix; intracellular membrane-bound organelle; membrane; cytoplasm; microtubule organizing center; nucleus. Molecular Function: DNA helicase activity; protein binding; ATPase activity; ATP binding. Biological Process: regulation of transcription from RNA polymerase II promoter; mitosis; nucleosome assembly; establishment and/or maintenance of chromatin architecture; DNA replication-independent nucleosome assembly at centromere; transcription, DNA-dependent; cell division; regulation of growth; spermatogenesis; DNA repair; DNA duplex unwinding; DNA recombination

size1 :

0.1 mg

price1 :

345 USD

more info or order :

MyBioSource product webpage