antibody

Mouse monoclonal antibody Anti-Human DDX48

MBS120199

0.1 mg

345 USD

monoclonal

mouse

nonconjugated

2256C1

western blot, dot blot

Antibody

Mouse monoclonal antibody Anti-Human DDX48

DDX48

Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 (DDX48); EIF4A3;NUK-34; hNMP265; KIAA0111;MGC10862; eIF4AIII

Homo sapiens eukaryotic translation initiation factor 4A3 (EIF4A3), mRNA; eukaryotic initiation factor 4A-III; ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; NMP 265; eIF-4A-III; eIF4A-III; eukaryotic initiation factor 4A-like NUK-34; eukaryotic translation initiation factor 4A, isoform 3; hNMP 265; nuclear matrix protein 265; eukaryotic translation initiation factor 4A3

DDX48

EIF4A3; EIF4A3; RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII

IF4A3_HUMAN

Monoclonal

IgG1

2256C1

Mouse

Human

1734

100 ug/ml (1.0 ml)

Dot Blot (DB)

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Sterility: Filtered through a 0.22 um membrane.

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [NCBI Entrez Gene Summary]

288915541

NP_055555.1

NM_014740

46,871 Da

Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Cytokine Signaling In Immune System Pathway (366171); Deadenylation Of MRNA Pathway (160994); Deadenylation-dependent MRNA Decay Pathway (160993); Exon Junction Complex (EJC) Pathway (470353); Exon Junction Complex (EJC) Pathway (890638); Gene Expression Pathway (105937); ISG15 Antiviral Mechanism Pathway (530761); Immune System Pathway (106386); Interferon Signaling Pathway (187103)

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq, Jul 2008]

DDX48: ATP-dependent RNA helicase. Component of a splicing- dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP- bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Belongs to the DEAD box helicase family. eIF4A subfamily. Protein type: Spliceosome; Helicase; RNA-binding; EC 3.6.4.13; RNA splicing. Chromosomal Location of Human Ortholog: 17q25.3. Cellular Component: nucleoplasm; membrane; cytoplasm; nuclear speck; cytosol. Molecular Function: mRNA binding; protein binding; ATP binding; poly(A) binding; ATP-dependent RNA helicase activity. Biological Process: nuclear mRNA splicing, via spliceosome; poly(A) tail shortening; mRNA transport; positive regulation of translation; negative regulation of translation; cytokine and chemokine mediated signaling pathway; mRNA catabolic process, nonsense-mediated decay; gene expression; embryonic cranial skeleton morphogenesis; rRNA processing; mRNA catabolic process, deadenylation-dependent decay. Disease: Robin Sequence With Cleft Mandible And Limb Anomalies

0.1 mg

345 USD