antibody

Mouse monoclonal antibody Anti-Human DDB2

MBS120183

0.1 mg

345 USD

monoclonal

mouse

nonconjugated

2246C4a

human, mouse

western blot, immunocytochemistry

Antibody

Mouse monoclonal antibody Anti-Human DDB2

DDB2

Homo sapiens damage-specific DNA binding protein 2, 48kDa (DDB2),?mRNA; FLJ34321

Homo sapiens damage-specific DNA binding protein 2, 48kDa (DDB2), mRNA; DNA damage-binding protein 2; DNA damage-binding protein 2; DDB p48 subunit; UV-DDB 2; UV-damaged DNA-binding protein 2; damage-specific DNA-binding protein 2; xeroderma pigmentosum group E protein; damage-specific DNA binding protein 2, 48kDa; DDB p48 subunit; DDBb; Damage-specific DNA-binding protein 2; UV-damaged DNA-binding protein 2; UV-DDB 2

DDB2

DDB2; DDB2; DDBB; UV-DDB2

DDB2_HUMAN

Monoclonal

IgG1

2246C4a

Mouse

Human

1870

100 ug/ml (1.0 ml)

Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB)

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Sterility: Filtered through a 0.22 um membrane.

Plays a role in DNA repair by forming with DDB1 the UV-damaged DNA-binding protein complex (UV-DDB). Binds to pyrimidine dimers. Component of the RBX1-CUL4-DDB2 ubiquitin ligase. Required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. [UniProtKB Function]

164419759

NP_000098.1

NM_000107

26,758 Da

Cul4-DDB1-DDB2 Complex Pathway (413452); Cul4-DDB1-DDB2 Complex Pathway (890608); DNA Repair Pathway (105837); DNA Damage Response Pathway (198788); Direct P53 Effectors Pathway (137939); Dual Incision Reaction In GG-NER Pathway (105887); Formation Of Incision Complex In GG-NER Pathway (105886); Global Genomic NER (GG-NER) Pathway (105884); Hepatitis B Pathway (694606); Nucleotide Excision Repair Pathway (105883)

This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

DDB2: Required for DNA repair. Binds to DDB1 to form the UV- damaged DNA-binding protein complex (the UV-DDB complex). The UV- DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1- CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4- ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair. Component of the UV-DDB complex which includes DDB1 and DDB2. The UV-DDB complex interacts with monoubiquitinated histone H2A and binds to XPC via the DDB2 subunit. Component of the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4- ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1), which includes CUL4A or CUL4B, DDB1, DDB2 and RBX1. DDB2 may function as the substrate recognition module within this complex. The DDB1- CUL4-ROC1 complex may associate with the COP9 signalosome, and this inhibits the E3 ubiquitin-protein ligase activity of the complex. A large number of other DCX complexes may also exist in which an alternate substrate targeting subunit replaces DDB2. These targeting subunits are generally known as DCAF (DDB1- and CUL4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Isoform D1 and isoform D2 do not interact with DDB1. Expression is induced in response to treatment with IR or UV and this requires p53/TP53 activity. Ubiquitously expressed; with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed. Belongs to the WD repeat DDB2/WDR76 family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: DNA repair, damage. Chromosomal Location of Human Ortholog: 11p12-p11. Cellular Component: nucleoplasm; protein complex; cell junction. Molecular Function: protein binding; DNA binding; ubiquitin-protein ligase activity; damaged DNA binding. Biological Process: protein autoubiquitination; protein polyubiquitination; nucleotide-excision repair; pyrimidine dimer repair; nucleotide-excision repair, DNA damage removal; DNA repair; response to UV. Disease: Xeroderma Pigmentosum, Complementation Group E

0.1 mg

345 USD