antibody

Mouse monoclonal antibody Anti-Human CUL3

MBS120162

0.1 mg

345 USD

monoclonal

mouse

nonconjugated

2236C1a

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

Antibody

Mouse monoclonal antibody Anti-Human CUL3

CUL3

Homo sapiens cullin 3 (CUL3)

Homo sapiens cullin 3 (CUL3), transcript variant 1, mRNA; Cullin-3; cullin-3; cullin 3

CUL3

CUL3; CUL3; CUL-3; PHA2E

CUL3_HUMAN

Monoclonal

IgG1

2236C1a

Mouse

Human

6790

Mouse monoclonal anti-human CUL3 antibody in PBS (3.0 mM KCl, 1.5mM KH2PO4, 140mM NaCl, 8.0mM Na2HPO4 (pH7.4) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).

100 ug/ml (1.0 ml)

Store at 2-8 degree C for up to one year. We recommended storeing at -20 degree C for long-term storage. Avoid repeat freezing and thawing cycles.

Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)

WB: 0.2-2ug/ml. IP: 100-500ug/sample. FC: 0.5-2ug/sample. ICC: 2-100 ug/ml

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Sterility: Filtered through a 0.22 um membrane. Disposal: This antibody solution contains sodium azide (NaN3) as a preaservative. There is a potential hazard taht NaN3 reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. Safety warnings and precautions: Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly techniques and that it is handled with care pursuant to be principles of good laboratory practice. All chemicalas are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off.

Involved in ubiquitination of cyclin E and, in complex with RBX1, of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Seems not be part of a SCF complex with SKP1. In complex with SPOP, involved in ubiquitination of both BMI1/PCGF4 and H2AFY. [UniProtKB Function]

380714661

NP_003581.1

NM_003590

ATF-2 Transcription Factor Network Pathway (138006); Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Aurora B Signaling Pathway (138080); Canonical Wnt Signaling Pathway (138032); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cul3-SPOP Complex Pathway (413451); Cul3-SPOP Complex Pathway (890607); Disease Pathway (530764); Hedgehog 'on' State Pathway (1127542)

This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

CUL3: a core component of multiple cullin-RING-based BCR (BTB- CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with CAND1. The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. SPOP is involved in ubiquitination of BMI1, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. BCR(KLHL12) is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31A or -B. BCR(KLHL3) acts as a regulator of ion transport in the distal nephron; possibly by mediating ubiquitination of SLC12A3/NCC. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Forms neddylation-dependent homodimers. Component of multiple BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes formed of CUL3, RBX1 and a variable BTB domain-containing protein acting as both, adapter to cullin and substrate recognition subunit. The BCR complex may be active as a heterodimeric complex, in which NEDD8, covalently attached to one CUL3 molecule, binds to the C-terminus of a second CUL3 molecule. Interacts with RBX1, RNF7, CYCE and CAND1. Part of the BCR(SPOP) containing SPOP. Part of the probable BCR(KLHL9-KLHL13) complex with BTB domain proteins KLHL9 and KLHL13. Part of the BCR(KBTBD10) complex containing KBTBD10. Component of the BCR(KLHL12) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL12 and RBX1. Component of the BCR(KLHL3) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL3 and RBX1 (Probable). Part of the BCR(ENC1) complex containing ENC1. Part of a complex consisting of BMI1, CUL3 and SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with KCTD5, KLHL9, KLHL13, GAN, ZBTB16, KLHL21, KLHL3, KLHL15, KLHL20, C16orf44, GMCL1P1, BTBD1. Part of a complex that contains CUL3, RBX1 and GAN. Interacts (via BTB domain) with KLHL17; the interaction regulates surface GRIK2 expression. Widely expressed. Belongs to the cullin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation; Ubiquitin ligase; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 2q36.2. Cellular Component: nucleoplasm; Golgi membrane; membrane; polar microtubule; cytosol. Molecular Function: cyclin binding; protein binding; protein homodimerization activity; protein heterodimerization activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; POZ domain binding. Biological Process: protein monoubiquitination; integrin-mediated signaling pathway; positive regulation of cytokinesis; proteasomal ubiquitin-dependent protein catabolic process; ER to Golgi vesicle-mediated transport; cell migration; Wnt receptor signaling pathway; protein polyubiquitination; stem cell division; protein ubiquitination; gastrulation; mitotic metaphase plate congression; embryonic cleavage; COPII coating of Golgi vesicle; cyclin catabolic process; negative regulation of Rho protein signal transduction; positive regulation of cell proliferation; positive regulation of mitotic metaphase/anaphase transition; stress fiber formation; trophectodermal cellular morphogenesis; cell cycle arrest; G1/S transition of mitotic cell cycle. Disease: Pseudohypoaldosteronism, Type Iie

0.1 mg

345 USD