antibody

Mouse monoclonal antibody Anti-Human XBP1

MBS120080

0.1 mg

345 USD

monoclonal

mouse

nonconjugated

XBP1H6E5

western blot, dot blot

Antibody

Mouse monoclonal antibody Anti-Human XBP1

XBP1

X-box binding protein 1; XBP2; TREB5

Homo sapiens X-box binding protein 1 (XBP1), transcript variant 1, mRNA; X-box-binding protein 1; X-box-binding protein 1; tax-responsive element-binding protein 5; X-box binding protein 1; Tax-responsive element-binding protein 5

XBP1

XBP1; XBP1; XBP2; TREB5; XBP-1; TREB-5

XBP1_HUMAN

Monoclonal

IgG1

XBP1H6E5

Mouse

Human

1820

100 ug/ml (1.0 ml)

Dot Blot (DB)

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Sterility: Filtered through a 0.22 um membrane.

This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein and was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has also been found that the same agents that cause an ER stress response, the unfolded protein response (UPR), also increase transcription of this gene. This increase in transcription requires an ER stress response consensus binding element in the promoter. In the UPR pathway, two isoforms of the protein are made. The function of the shorter isoform (XBP-1U) has not been determined, while the longer isoform (XBP-1S) is the active isoform. The longer isoform is generated by a process in which two adjecent stem loop structures in the mRNA are recognized by IRE1, resulting in the loss of 26 nt from the mRNA and a frameshift in the CDS. Finally, a pseudogene has been identified and localized to chromosome 5. [NCBI Entrez Gene Summary]

172072591

NP_005071.2

NM_005080

40,148 Da

ATF6-alpha Activates Chaperone Genes Pathway (530770); ATF6-alpha Activates Chaperones Pathway (105905); FOXA1 Transcription Factor Network Pathway (137979); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); IRE1alpha Activates Chaperones Pathway (105906); Insulin Signaling Pathway (198845); Metabolism Of Proteins Pathway (106230); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862188); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862314)

This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008]

XBP1: a transcription factor essential for hepatocyte growth, the differentiation of plasma cells, immunoglobulin secretion, and the unfolded protein response (UPR). XBP1 mRNA is spliced by IRE1 during the UPR to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Only the spliced form of XBP1 can activate the UPR efficiently. Activates UPR target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5 -GATGACGTG[TG]N(3)[AT]T-3 , and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress. Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7). Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. Two human isoforms are produced by alternative splicing. Isoform 1 is also known as XBP-1U. Isoform 2, also known as XBP-1S, is produced by IRE1 in response to endoplasmic reticulum stress. IRE1 cleaves a 26-bp fragment causing a frameshift of the mRNA transcript. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 22q12.1 22q12. Cellular Component: nucleoplasm; endoplasmic reticulum membrane; endoplasmic reticulum; cytoplasm; integral to membrane; integral to endoplasmic reticulum membrane; cytosol; nucleus. Molecular Function: protein binding; protein homodimerization activity; protease binding; DNA binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; estrogen receptor binding; protein kinase binding; transcription factor activity. Biological Process: ubiquitin-dependent protein catabolic process; transcription from RNA polymerase II promoter; phosphoinositide 3-kinase cascade; muscle development; apoptosis; positive regulation of transcription of target genes involved in unfolded protein response; exocrine pancreas development; regulation of protein stability; negative regulation of transcription from RNA polymerase II promoter; cellular response to glucose starvation; protein transport; serotonin secretion, neurotransmission; positive regulation of MHC class II biosynthetic process; positive regulation of transcription factor import into nucleus; angiogenesis; cell growth; response to electrical stimulus; fatty acid biosynthetic process; positive regulation of TOR signaling pathway; positive regulation of autophagy; response to drug; positive regulation of histone methylation; protein destabilization; unfolded protein response; organelle organization and biogenesis; cellular response to nutrient; positive regulation of immunoglobulin secretion; liver development; cholesterol homeostasis; positive regulation of immunoglobulin production; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; cellular response to insulin stimulus; positive regulation of T cell differentiation; endothelial cell proliferation; fatty acid homeostasis; positive regulation of B cell differentiation; autophagy; positive regulation of fat cell differentiation; neuron development; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; sterol homeostasis; vascular endothelial growth factor receptor signaling pathway; negative regulation of apoptosis. Disease: Major Affective Disorder 7

0.1 mg

345 USD