antibody

Mouse monoclonal antibody Anti-Human SMARCA4

MBS120065

0.1 mg

345 USD

monoclonal

mouse

nonconjugated

SMAR9G1F9

western blot, dot blot

Antibody

Mouse monoclonal antibody Anti-Human SMARCA4

SMARCA4

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4); BRG1; BAF190; SNF2L4; SNF2LB; hSNF2b;SNF2-BETA

Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), transcript variant 3, mRNA; Transcription activator BRG1; transcription activator BRG1; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BRM/SWI2-related gene 1; SNF2-beta; SNF2-like 4; brahma protein-like 1; global transcription activator homologous sequence; homeotic gene regulator; mitotic growth and transcription activator; nuclear protein GRB1; protein BRG-1; protein brahma homolog 1; sucrose nonfermenting-like 4; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; SNF2-beta; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4

SMARCA4

SMARCA4; SMARCA4; BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A

SMCA4_HUMAN

Monoclonal

IgG1

SMAR9G1F9

Mouse

Human

5703

100 ug/ml (1.0 ml)

Dot Blot (DB)

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Sterility: Filtered through a 0.22 um membrane.

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined. [NCBI Entrez Gene Summary]

192807310

NP_003063.2

NM_003072

181,261 Da

Chromatin Modifying Enzymes Pathway (1127682); Chromatin Organization Pathway (1127681); Direct P53 Effectors Pathway (137939); Disease Pathway (530764); Glucocorticoid Receptor Regulatory Network Pathway (138014); Integrated Breast Cancer Pathway (219801); Prostate Cancer Pathway (755440); RMTs Methylate Histone Arginines Pathway (1127687); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1127632); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352)

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

SMARCA4: a member of the SWI/SNF nucleosome remodeling complex with ATP-dependent DNA helicase activity. SWI/SNF complexes are required for mammalian development and are mutated in ~20% of all human primary tumors. Mutated in 35% (13/37) of NSCLC and 5% (1/19) of SCLC human lung cancer cell lines . A component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. Belongs to the neural progenitor-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). Component of the BAF53 complex which acetylates histone H4 and H2A within nucleosomes. Transcriptional coactivator of nuclear hormone receptors. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription, and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. SMARCA4, in consort with ZEB1, represses the transcription of E-cadherin and promotes the epithelial-to-mesenchymal transisition (EMT). SMARCA4 is required for stem cell maintenance in the mouse intestinal epithelium. Its loss inhibits aberrant Wnt-signalling and prevents tumorigenesis in the mouse small intestine. SMARCA4 mutations are the cause of a familial cancer syndrome predisposing to renal or extrarenal malignant rhabdoid tumors and to a variety of tumors of the central nervous system, including choroid plexus carcinoma, medulloblastoma, and central primitive neuroectodermal tumors. Rhabdoid tumors are the most aggressive and lethal malignancies occurring in early childhood. Belongs to the SNF2/RAD54 helicase family. The human protein includes 5 isoforms produced by alternative splicing. Protein type: EC 3.6.4.-; Cell cycle regulation; EC 3.6.1.-; Nuclear receptor co-regulator; Tumor suppressor; Transcription, coactivator/corepressor; Helicase. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: nucleoplasm; heterochromatin; extracellular space; SWI/SNF complex; protein complex; perichromatin fibrils; membrane; nuclear chromatin; nucleolus; nucleus. Molecular Function: DNA-dependent ATPase activity; protein binding; nucleosomal DNA binding; androgen receptor binding; Tat protein binding; p53 binding; transcription coactivator activity; protein N-terminus binding; helicase activity; transcription corepressor activity; ATP binding. Biological Process: establishment and/or maintenance of chromatin architecture; extracellular matrix organization and biogenesis; glial cell fate determination; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter, mitotic; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; embryonic organ morphogenesis; epidermis morphogenesis; forebrain development; blastocyst hatching; blastocyst growth; vasculogenesis; positive regulation of Wnt receptor signaling pathway; hindbrain development; positive regulation of DNA binding; transcription, DNA-dependent; stem cell maintenance; liver development; regulation of transcription from RNA polymerase II promoter; chromatin remodeling; keratinocyte differentiation; cytosine methylation within a CG sequence; nucleosome disassembly; methylation-dependent chromatin silencing; ATP-dependent chromatin remodeling; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of transcription, DNA-dependent. Disease: Mental Retardation, Autosomal Dominant 16; Rhabdoid Tumor Predisposition Syndrome 2

0.1 mg

345 USD