protein

Recombinant Saccharomyces cerevisiae Site-specific recombinase Flp (FLP1)

MBS1122843

0.01 mg (E-Coli)

200 USD

Recombinant Protein

Recombinant Saccharomyces cerevisiae Site-specific recombinase Flp (FLP1)

[Site-specific recombinase Flp (FLP1)]

[Site-specific recombinase Flp; FLP; Protein Able]

[recombinase (FLP); Site-specific recombinase Flp; Protein Able]

[FLP1]

[FLP1; R0010W]

[FLP1; FLP]

FLP_YEAST

E Coli or Yeast or Baculovirus or Mammalian Cell

[1-423. Full Length]

MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELT
YLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQ
FKYKTQKATILEASLKKLIPAWEFTIIPYYGQKHQSDIT
DIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWE
ITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDI
KNVDPKSFKLVQNKYLGVIIQCLVTETKTSVSRHIYFFS
ARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQ
LLKDNLVRSYNKALKKNAPYSIFAIKNGPKSHIGRHLMT
SFLSMKGLTELTNVVGNWSDKRASAVARTTYTHQITAIP
DHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI

>=90%

Liquid containing glycerol

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

SDS-PAGE

Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Part of the plasmid amplification system, which corrects any decrease in copy number caused by a rare missegregation event. Catalyzes the recombination between the large inverted repetitions of the 2-micron plasmid during plasmid replication. This recombination event changes the direction of one of the two replication forks in the bidirectionally replicating molecule, effectively resulting in multiple rounds of replication from a single initiation event. Binds specifically to the FLP recognition target (FRT) site where it induces DNA to bend. Three types of bend exist. Type I is approximately 60 degrees and results from 1 FLP molecule binding to 1 symmetry element. Type II is >144 degrees and results from FLP molecules binding to symmetry elements a and b. Type III is approximately 65 degrees and results from FLP molecules binding to symmetry elements b and c.

11466068

NP_040488.1

NC_001398.1

P03870

48,620 Da

Flp recombinase: Part of the plasmid amplification system, which corrects any decrease in copy number caused by a rare missegregation event. Catalyzes the recombination between the large inverted repetitions of the 2-micron plasmid during plasmid replication. This recombination event changes the direction of one of the two replication forks in the bidirectionally replicating molecule, effectively resulting in multiple rounds of replication from a single initiation event. Binds specifically to the FLP recognition target (FRT) site where it induces DNA to bend. Three types of bend exist. Type I is approximately 60 degrees and results from 1 FLP molecule binding to 1 symmetry element. Type II is 144 degrees and results from FLP molecules binding to symmetry elements a and b. Type III is approximately 65 degrees and results from FLP molecules binding to symmetry elements b and c

0.01 mg (E-Coli)

200 USD

0.05 mg (E-Coli)

260

0.1 mg (E-Coli)

430

0.2 mg (E-Coli)

685

0.05 mg (Yeast)

940

0.5 mg (E-Coli)

1125

0.05 mg (Baculovirus)

1150

0.2 mg (Yeast)

1260

0.05 mg (Mammalian-Cell)

1390

0.5 mg (Yeast)

1425

0.1 mg (Baculovirus)

1660

1 mg (E-Coli)

1725

0.5 mg (Baculovirus)

2155

0.1 mg (Mammalian-Cell)

2250

1 mg (Yeast)

2255

1 mg (Baculovirus)

3330