protein

Recombinant Human papillomavirus type 16 Minor capsid protein L2

MBS1089949

0.05 mg (E-Coli)

190 USD

Recombinant Protein

Recombinant Human papillomavirus type 16 Minor capsid protein L2

papillomavirus type 16 Minor capsid protein L2

minor capsid protein; Minor capsid protein L2; minor capsid protein

L2

L2; L2

VL2_HPV16

E Coli or Yeast or Baculovirus or Mammalian Cell

1-473

473

MRHKRSAKRTKRASATQLYKTCKQAGTCPPDIIPKVEGK
TIAEQILQYGSMGVFFGGLGIGTGSGTGGRTGYIPLGTR
PPTATDTLAPVRPPLTVDPVGPSDPSIVSLVEETSFIDA
GAPTSVPSIPPDVSGFSITTSTDTTPAILDINNTVTTVT
THNNPTFTDPSVLQPPTPAETGGHFTLSSSTISTHNYEE
IPMDTFIVSTNPNTVTSSTPIPGSRPVARLGLYSRTTQQ
VKVVDPAFVTTPTKLITYDNP

Greater than 90% as determined by SDS-PAGE.

Liquid containing glycerol; lyophilization may be available upon request.

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

Minor protein of the capsid that localizes along the inner surface of the virion, within the central cavities beneath the L1 pentamers. Plays a role in capsid stabilization through interaction with the major capsid protein L1. Once the virion enters the host cell, escorts the genomic DNA into the nucleus, in particular by promoting virion endosomal escape. It is involved, through its interaction with host dynein, in the intracellular microtubule-dependent transport of viral capsid toward the nucleus. Mediates the viral genome import into the nucleus through binding to host importins. Once within the nucleus, L2 localizes viral genomes to PML bodies in order to activate early gene expression for establishment of infection. Later on, promotes late gene expression by interacting with the viral E2 protein and by inhibiting its transcriptional activation functions. During virion assembly, encapsidates the genome by direct interaction with the viral DNA.

Human papillomavirus type 16 DNA sequence.Seedorf K., Krammer G., Durst M., Suhai S., Rowekamp W.G.Virology 145:181-185(1985) Cloning and sequencing of non-European human papillomavirus (HPV) variant complete genomes from cervicovaginal cells by an overlapping PCR method.Terai M., Fu L., Ma Z., Burk R.D. Interaction of human papillomavirus type 16 L2 with cellular proteins identification of novel nuclear body-associated proteins.Goernemann J., Hofmann T.G., Will H., Mueller M.Virology 303:69-78(2002)Interaction of human papillomavirus (HPV)type 16 capsid proteins with HPV DNA requires an intact L2 N-terminal sequence.Zhou J., Sun X.Y., Louis K., Frazer I.H.J. Virol. 68:619-625(1994) Human papillomavirus type 16 capsid proteins produced from recombinant Semliki Forest virus assemble into virus-like particles.Heino P., Dillner J., Schwartz S.Virology 214:349-359(1995) Nuclear translocation of papillomavirus minor capsid protein L2 requires Hsc70.Florin L., Becker K.A., Sapp C., Lambert C., Sirma H., Muller M., Streeck R.E., Sapp M.J. Virol. 78:5546-5553(2004) The l2 minor capsid protein of human papillomavirus type 16 interacts with a network of nuclear import receptors.Darshan M.S., Lucchi J., Harding E., Moroianu J.J. Virol. 78:12179-12188(2004) Human papillomavirus 16 L2 inhibits the transcriptional activation function, but not the DNA replication function, of HPV-16 E2.Okoye A., Cordano P., Taylor E.R., Morgan I.M., Everett R., Campo M.S.Virus Res. 108:1-14(2005) Expression pattern and subcellular localization of human papillomavirus minor capsid protein L2.Lin Z., Yemelyanova A.V., Gambhira R., Jagu S., Meyers C., Kirnbauer R., Ronnett B.M., Gravitt P.E., Roden R.B.Am. J. Pathol. 174:136-143(2009) Two highly conserved cysteine residues in HPV16 L2 form an intramolecular disulfide bond and are critical for infectivity in human keratinocytes.Campos S.K., Ozbun M.A.PLoS ONE 4:E4463-E4463(2009) Identification of the dynein light chains required for human papillomavirus infection.Schneider M.A., Spoden G.A., Florin L., Lambert C.Cell. Microbiol. 13:32-46(2011)

9627107

NP_041331.1

NC_001526.2

P03107

66.7kD

0.05 mg (E-Coli)

190 USD

0.2 mg (E-Coli)

460

0.5 mg (E-Coli)

750

1 mg (E-Coli)

1180

0.05 mg (Baculovirus)

1255