catalog number :
MBS108890
products type :
ELISA Kit
products full name :
Plant Alternative Oxidase (AOX) ELISA Kit
products short name :
[Alternative Oxidase (AOX)]
products gene name :
[AOX]
specificity :
No significant cross-reactivity or interference between Plant AOX and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Plant Tissue Homogenate and other Plant Biological Fluids. Assay Type: Sandwich. Detection Range: 1.25ng/ml-40ng/ml. Sensitivity: 0.1ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated AOX concentrations in plant tissue homogenate and other plant biological fluids. Using Purified Plant AOX antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AOX and AOX antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AOX in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) are less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated AOX concentrations in plant tissue homogenate and other plant biological fluids. Using Purified Plant AOX antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AOX and AOX antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AOX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
size4 :
10x96-Strip-Wells