catalog number :
MBS1047376
products type :
Recombinant Protein
products full name :
Recombinant Bacillus subtilis (strain 168) Phosphocarrier protein HPr
products short name :
Phosphocarrier protein HPr
products name syn :
Histidine-containing protein
other names :
phosphocarrier protein HPr; Phosphocarrier protein HPr; phosphocarrier protein HPr; Histidine-containing protein
products gene name :
ptsH
other gene names :
ptsH; ptsH
uniprot entry name :
PTHP_BACSU
host :
E Coli or Yeast or Baculovirus or Mammalian Cell
sequence positions :
Feb-88
sequence :
AQKTFKVTADSGIHARPATVLVQTASKYDADVNLEYNGK
TVNLKSIMGVMSLGIAKGAEITISASGADENDALNALEE
TMKSEGLGE
purity :
Greater than 90% as determined by SDS-PAGE.
form :
Liquid containing glycerol; lyophilization may be available upon request.
storage stability :
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
other info2 :
Species: Bacillus subtilis (strain 168)
products description :
General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the permease (enzymes II/III).
P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbon catabolite repression (CCR), a mechanism that allows bacteria to coordinate and optimize the utilization of available carbon sources. P-Ser-HPr also plays a role in inducer exclusion, in which it probably interacts with several non-PTS permeases and inhibits their transport activity.
products references :
Phosphoenolpyruvate:sugar phosphotransferase system of Bacillus subtilis: nucleotide sequence of ptsX, ptsH and the 5'-end of ptsI and evidence for a ptsHI operon."
Gonzy-Treboul G., Zagorec M., Rain-Guion M.-C., Steinmetz M.
Mol. Microbiol. 3:103-112(1989)
ncbi acc num :
NP_389273.1
ncbi gb acc num :
NC_000964.3
ncbi mol weight :
25.05kD
size5 :
0.05 mg (Baculovirus)