catalog number :
MBS1045029
products type :
Recombinant Protein
products full name :
Recombinant Escherichia coli Cytidylate kinase
products short name :
Cytidylate kinase
products name syn :
Cytidine monophosphate kinase; CMP kinase; Protein MssA; p25
other names :
cytidylate kinase; Cytidylate kinase; cytidylate kinase; Cytidine monophosphate kinase; CMP kinase; Protein MssA; p25
other gene names :
cmk; cmk; ECK0901; JW0893; mssA; ycaF; ycaG; mssA; ycaF; ycaG; CK; CMP kinase
uniprot entry name :
KCY_ECOLI
host :
E Coli or Yeast or Baculovirus or Mammalian Cell
sequence positions :
1-227
sequence :
MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAI
YRVLALAALHHHVDVASEDALVPLASHLDVRFVSTNGNL
EVILEGEDVSGEIRTQEVANAASQVAAFPRVREALLRRQ
RAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAH
RRMLQLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVP
AADALVLDSTTLSIEQVIEKALQYARQKLALA
purity :
Greater than 90% as determined by SDS-PAGE.
form :
Liquid containing glycerol; lyophilization may be available upon request.
storage stability :
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
products description :
ATP, dATP, and GTP are equally effective as phosphate donors. CMP and dCMP are the best phosphate acceptors.
products references :
Transcriptional organization of the rpsA operon of Escherichia coli.Pedersen S., Skouv J., Kajitani M., Ishihama A.Mol. Gen. Genet. 196:135-140(1984)
A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.DNA Res. 3:137-155(1996)
The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997)
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006)
The DNA sequence of the gene rpsA of Escherichia coli coding for ribosomal protein S1.Schnier J., Isono K.Nucleic Acids Res. 10:1857-1865(1982)
Multicopy suppressors, mssA and mssB, of an smbA mutation of Escherichia coli.Yamanaka K., Ogura T., Koonin E.V., Niki H., Hiraga S.Mol. Gen. Genet. 243:9-16(1994)
The cmk gene encoding cytidine monophosphate kinase is located in the rpsA operon and is required for normal replication rate in Escherichia coli.Fricke J., Neuhard J., Kelln R.A., Pedersen S.J. Bacteriol. 177:517-523(1995)
Structural and catalytic properties of CMP kinase from Bacillus subtilis
a comparative analysis with the homologous enzyme from Escherichia coli.Schultz C.P., Ylisastigui-Pons L., Serina L., Sakamoto H., Mantsch H.H., Neuhard J., Barzu O., Gilles A.M.Arch. Biochem. Biophys. 340:144-153(1997)
Structures of Escherichia coli CMP kinase alone and in complex with CDP
a new fold of the nucleoside monophosphate binding domain and insights into cytosine nucleotide specificity.Briozzo P., Golinelli-Pimpaneau B., Gilles A.M., Gaucher J.F., Burlacu-Miron S., Sakamoto H., Janin J., Barzu O.Structure 6:1517-1527(1998)
ncbi acc num :
NP_415430.1
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
40.73kD
ncbi pathways :
CMP Phosphorylation Pathway (782453); CMP Phosphorylation Pathway (908070); Metabolic Pathways (131985); Pyrimidine Metabolism Pathway (1023); Pyrimidine Metabolism Pathway (309); Pyrimidine Ribonucleotide Biosynthesis, UMP = UDP/UTP,CDP/CTP Pathway (405191); Pyrimidine Ribonucleotide Biosynthesis, UMP = UDP/UTP,CDP/CTP Pathway (468245); Pyrimidine Deoxyribonucleotide Phosphorylation Pathway (782452); Pyrimidine Deoxyribonucleotide Phosphorylation Pathway (907959); Salvage Pathways Of Pyrimidine Ribonucleotides (120)
size4 :
0.05 mg (Baculovirus)