catalog number :
MBS1042315
products type :
Recombinant Protein
products full name :
Recombinant Escherichia coli Glutamate--cysteine ligase
products short name :
Glutamate--cysteine ligase
products name syn :
Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase
other names :
glutamate-cysteine ligase; Glutamate--cysteine ligase; glutamate-cysteine ligase; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase
products gene name :
gshA
other gene names :
gshA; gshA; ECK2683; gshI; JW2663; gsh-I; GCS
uniprot entry name :
GSH1_ECOLI
host :
E Coli or Yeast or Baculovirus or Mammalian Cell
sequence positions :
1-518,Full length
sequence :
MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTL
ATTGHPEALGSALTHKWITTDFAEALLEFITPVDGDIEH
MLTFMRDLHRYTARNMGDERMWPLSMPCYIAEGQDIELA
QYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLP
MAFWQAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIP
YLFGASPAICSSFLQGKPTSLPFEKTECGMYYLPYATSL
RLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEE
YAKIGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSG
ESPSDALLRGGIEYIEVRSLDINPFSPIGVDEQQVRFLD
LFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLT
LGIGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAY
QKVCDELVACFDNPDLTFSARILRSMIDTGIGGTGKAFA
EAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADT
EPFAVWLEKHA
purity :
Greater than 90% as determined by SDS-PAGE.
form :
Liquid containing glycerol; lyophilization may be available upon request.
storage stability :
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
products references :
The nucleotide sequence of the gene for gamma-glutamylcysteine synthetase of Escherichia coli.Watanabe K., Yamano Y., Murata K., Kimura A.Nucleic Acids Res. 14:4393-4400(1986)
Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.DNA Res. 4:91-113(1997)
The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997)
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006)
ncbi acc num :
NP_417173.1
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
74.23kD
ncbi pathways :
Cysteine And Methionine Metabolism Pathway (103425); Cysteine And Methionine Metabolism Pathway (103421); Glutathione Biosynthesis, Glutamate = Glutathione Pathway (405204); Glutathione Biosynthesis, Glutamate = Glutathione Pathway (468307); Glutathione Metabolism Pathway (1050); Glutathione Metabolism Pathway (343); Metabolic Pathways (131985); Glutathione Biosynthesis Pathway (82); Glutathione Biosynthesis Pathway (138446)
size4 :
0.05 mg (Baculovirus)