catalog number :
MBS103237
products type :
ELISA Kit
products full name :
Monkey Phosphorylated Smad 2 ELISA Kit
products short name :
[Phosphorylated Smad 2]
other names :
[pS2; Trefoil factor 1; trefoil factor 1; protein pS2; polypeptide P1.A; gastrointestinal trefoil protein pS2; breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; trefoil factor 1; Breast cancer estrogen-inducible protein; PNR-2; Polypeptide P1.A; hP1.A; Protein pS2]
products gene name :
[PS2]
other gene names :
[TFF1; TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21; BCEI; PS2; hP1.A]
uniprot entry name :
TFF1_HUMAN
specificity :
No significant cross-reactivity or interference between Monkey PS2 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PS2 concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey PS2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PS2 and PS2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PS2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PS2 concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey PS2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PS2 and PS2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PS2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
CAA28695.1
ncbi mol weight :
9,150 Da
ncbi pathways :
FOXA1 Transcription Factor Network Pathway (137979); Integrated Pancreatic Cancer Pathway (711360)
ncbi summary :
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
uniprot summary :
TFF1: Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: extracellular space. Molecular Function: protein binding; growth factor activity. Biological Process: negative regulation of cell proliferation; response to peptide hormone stimulus; maintenance of gastrointestinal epithelium; carbohydrate metabolic process; digestion; cell differentiation; response to iron ion; response to estradiol stimulus
size4 :
10x96-Strip-Wells