catalog number :
MBS102294
products type :
ELISA Kit
products full name :
Porcine Neutrophil Gelatinase Associated Lipocalin ELISA Kit
products short name :
Neutrophil Gelatinase Associated Lipocalin
products gene name :
NGAL
specificity :
No significant cross-reactivity or interference between Porcine NGAL and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 15.6 ng/ml - 500 ng/ml. Sensitivity: 2.0 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NGAL concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine NGAL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NGAL and NGAL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NGAL in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) are less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NGAL concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine NGAL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NGAL and NGAL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NGAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.