Rat G Protein Coupled Receptor 120 ELISA Kit | MyBioSource MBS102225 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat G Protein Coupled Receptor 120 ELISA Kit

catalog :

MBS102225

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS102225

products type :

ELISA Kit

products full name :

Rat G Protein Coupled Receptor 120 ELISA Kit

products short name :

G Protein Coupled Receptor 120

other names :

GPR120 protein; Free fatty acid receptor 4; free fatty acid receptor 4; omega-3 fatty acid receptor 1; G-protein coupled receptor 120; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4; free fatty acid receptor 4; G-protein coupled receptor 120; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4; Omega-3 fatty acid receptor 1

products gene name :

GPR120

other gene names :

FFAR4; FFAR4; GT01; PGR4; BMIQ10; GPR120; GPR129; O3FAR1; GPR120; GPR129; O3FAR1; PGR4

uniprot entry name :

FFAR4_HUMAN

reactivity :

Rat

sequence length :

361

specificity :

No significant cross-reactivity or interference between Rat GPR120 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GPR120 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GPR120 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GPR120 and GPR120 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GPR120 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) are less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GPR120 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GPR120 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GPR120 and GPR120 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GPR120 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

72533526

ncbi acc num :

AAI01176.1

uniprot acc num :

Q5NUL3

ncbi mol weight :

42,241 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Free Fatty Acid Receptors Pathway (161023); GPCR Ligand Binding Pathway (161020); Incretin Synthesis, Secretion, And Inactivation Pathway (187170); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356); Synthesis, Secretion, And Inactivation Of Glucagon-like Peptide-1 (GLP-1) Pathway (187171)

ncbi summary :

This gene encodes a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

uniprot summary :

GPR120: Receptor for medium and long-chain free fatty acid (FAA). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti- inflammatory effects particularly in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1 dependent effect but independent of G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediate the taste of fatty acids. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Lipid-binding; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral. Chromosomal Location of Human Ortholog: 10q23.33. Cellular Component: endocytic vesicle; integral to plasma membrane; plasma membrane. Molecular Function: peptide binding; fatty acid binding; taste receptor activity. Biological Process: hormone secretion; G-protein coupled receptor protein signaling pathway; negative regulation of inflammatory response; negative regulation of cytokine secretion; detection of chemical stimulus involved in sensory perception of taste; cellular response to hormone stimulus; negative regulation of apoptosis. Disease: Body Mass Index Quantitative Trait Locus 10

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage