product summary
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company name :
MyBioSource
product type :
protein
product name :
Recombinant African cassava mosaic virus Replication-associated protein
catalog :
MBS1010748
quantity :
0.05 mg (E-Coli)
price :
190 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS1010748
products type :
Recombinant Protein
products full name :
Recombinant African cassava mosaic virus Replication-associated protein
products short name :
Replication-associated protein
products name syn :
40.4 kDa protein; Protein AC1; Protein AL1
other names :
Replication-associated protein; Replication-associated protein; 40.4 kDa protein; Protein AC1; Protein AL1
products gene name :
AC1
products gene name syn :
AL1
other gene names :
AC1; Rep
uniprot entry name :
REP_CLVK
host :
E Coli or Yeast or Baculovirus or Mammalian Cell
sequence positions :
1-358
sequence length :
358
sequence :
MRTPRFRIQAKNVFLTYPKCSIPKEHLLSFIQTLSLQSN
PKFIKICRELHQNGEPHLHALIQFEGKITITNNRLFDCV
HPSCSTSFHPNIQGAKSSSDVKSYLDKDGDTVEWGQFQI
DGRSARGGQQSANDAYAKALNSGSKSEALNVIRELVPKD
FVLQFHNLNSNLDRIFQEPPAPYVSPFPCSSFDQVPVEI
EEWVADNVRDSAARPWRPNSIVIEGDSRTGKTIWARSLG
PHNYLCGHLDLSPKVFNNAAW
purity :
Greater than 90% as determined by SDS-PAGE.
form :
Liquid containing glycerol; lyophilization may be available upon request.
storage stability :
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
products description :
Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all giniviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.
products references :
Nucleotide sequence of cassava latent virus DNA.Stanley J., Gay M.R.Nature 301:260-262(1983)
ncbi gi num :
137408
ncbi acc num :
P14982.1
uniprot acc num :
P14982
ncbi mol weight :
56.32kD
size1 :
0.05 mg (E-Coli)
price1 :
190 USD
size2 :
0.05 mg (Yeast)
price2 :
190
size3 :
0.2 mg (E-Coli)
price3 :
460
size4 :
0.2 mg (Yeast)
price4 :
460
size5 :
0.5 mg (Yeast)
price5 :
750
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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