ELISA/assay

Canine Wingless Type MMTV Integration Site Family, Member 5A ELISA Kit

MBS099760

48-Strip-Wells

435 USD

ELISA Kit

Canine Wingless Type MMTV Integration Site Family, Member 5A ELISA Kit

Wingless Type MMTV Integration Site Family, Member 5A

WNT5A

Canine

No significant cross-reactivity or interference between Canine WNT5A and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated WNT5A concentrations in Canine serum, plasma and other body fluids. Using Purified Canine WNT5A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add WNT5A and WNT5A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of WNT5A in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated WNT5A concentrations in Canine serum, plasma and other body fluids. Using Purified Canine WNT5A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add WNT5A and WNT5A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of WNT5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

48-Strip-Wells

435 USD

96-Strip-Wells

600