ELISA/assay

Rat Glucose-6-Phosphatase, Catalytic ELISA Kit

MBS097902

48-Strip-Wells

435 USD

ELISA Kit

Rat Glucose-6-Phosphatase, Catalytic ELISA Kit

Glucose-6-Phosphatase, Catalytic

Glucose-6-phosphatase; Glucose-6-phosphatase; glucose-6-phosphatase; G6Pase; G-6-Pase; G6Pase-alpha; glucose-6-phosphatase alpha; glucose-6-phosphatase, catalytic subunit; Glucose-6-phosphatase alpha

G6PC

G6PC; G6PC; G6PT; GSD1; G6PC1; GSD1a; G6PT; G-6-Pase; G6Pase

G6PC_HUMAN

Rat

357

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Undiluted original Rat body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Assay Type: Sandwich. Detection Range: 3.12 U/L - 100 U/L. Sensitivity: 1.0U/L.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of G6PC (hereafter termed "analyte") in undiluted original Rat body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

206729864

P35575.2

P35575

40,484 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Carbohydrate Digestion And Absorption Pathway (170720); Carbohydrate Digestion And Absorption Pathway (170654); DNA Damage Response (only ATM Dependent) Pathway (198827); Disease Pathway (530764); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); FoxO Family Signaling Pathway (138036)

Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]

G6PC: Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels. Defects in G6PC are the cause of glycogen storage disease type 1A (GSD1A). A metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. Belongs to the glucose-6-phosphatase family. Protein type: Carbohydrate Metabolism - starch and sucrose; Phosphatase (non-protein); Membrane protein, integral; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Transporter, SLC family; Endoplasmic reticulum; Membrane protein, multi-pass; Transporter; EC 3.1.3.9; Carbohydrate Metabolism - galactose. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: endoplasmic reticulum membrane; integral to membrane; integral to endoplasmic reticulum membrane. Molecular Function: glucose-6-phosphatase activity; phosphate binding; phosphotransferase activity, alcohol group as acceptor. Biological Process: steroid metabolic process; glycogen metabolic process; response to food; phosphorylated carbohydrate dephosphorylation; glycogen catabolic process; multicellular organism growth; glucose 6-phosphate metabolic process; pathogenesis; glucose transport; glucose homeostasis; gluconeogenesis; cholesterol homeostasis; triacylglycerol metabolic process; urate metabolic process; glucose-6-phosphate transport; regulation of gene expression; hexose transport; carbohydrate metabolic process; transmembrane transport. Disease: Glycogen Storage Disease Ia

48-Strip-Wells

435 USD

96-Strip-Wells

600