catalog number :
MBS097081
products type :
ELISA Kit
products full name :
Rat Wingless Type MMTV Integration Site Family, Member 10B ELISA Kit
products short name :
Wingless Type MMTV Integration Site Family, Member 10B
products gene name :
WNT10B
specificity :
No significant cross-reactivity or interference between Rat WNT10B and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated WNT10B concentrations in Rat serum, plasma and other body fluids. Using Purified Rat WNT10B antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add WNT10B and WNT10B antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of WNT10B in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated WNT10B concentrations in Rat serum, plasma and other body fluids. Using Purified Rat WNT10B antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add WNT10B and WNT10B antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of WNT10B in the samples is then determined by comparing the O.D. of the samples to the standard curve.