ELISA/assay

Mouse High Molecular Weight Kininogen ELISA Kit

MBS089195

48-Strip-Wells

470 USD

ELISA Kit

Mouse High Molecular Weight Kininogen ELISA Kit

[High Molecular Weight Kininogen]

[high molecular weight kininogen I isoform DeltaD5; Kininogen-1; kininogen-1; H-kininigen; L-kininogen; kininogen 1]

[HMWK]

[Kng1; Kng1; Kng; Kng]

KNG1_MOUSE

Mouse

480

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Body fluids, tissue homogenates, secretions or feces samples. Assay Type: Quantitative Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of HMWK (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

50082914

AAT70087.1

O08677

73,102 Da

ACE Inhibitor Pathway (198320); Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1001318); G Alpha (i) Signalling Events Pathway (1000973); G Alpha (q) Signalling Events Pathway (1000975); GPCR Downstream Signaling Pathway (1000971); GPCR Ligand Binding Pathway (1000941)

KNG1: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency). HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Inhibitor; Secreted; Contractile; Motility/polarity/chemotaxis; Secreted, signal peptide. Cellular Component: extracellular space; extracellular region. Molecular Function: protease inhibitor activity; cysteine protease inhibitor activity; receptor binding. Biological Process: hemostasis; negative regulation of peptidase activity; regulation of blood vessel size; inflammatory response; blood coagulation; vasodilation

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095