catalog number :
MBS083501
products type :
ELISA Kit
products full name :
Mouse Interleukin 1 Family, Member 9 ELISA Kit
products short name :
Interleukin 1 Family, Member 9
other names :
interleukin-36 gamma isoform 1; Interleukin-36 gamma; interleukin-36 gamma; IL-1 epsilon; IL-1-epsilon; IL-1(EPSILON); interleukin-1 epsilon; IL-1 related protein 2; IL-1-related protein 2; interleukin-1 homolog 1; interleukin-1 family member 9; interleukin 1 family, member 9; interleukin 1-related protein 2; interleukin 36, gamma; IL-1-related protein 2; IL-1RP2; Interleukin-1 epsilon; IL-1 epsilon; Interleukin-1 family member 9; IL-1F9; Interleukin-1 homolog 1
products gene name :
IL1F9
other gene names :
IL36G; IL36G; IL1E; IL1F9; IL1H1; IL-1F9; IL-1H1; IL1RP2; IL-1RP2; IL1E; IL1F9; IL1H1; IL1RP2; IL-1RP2; IL-1 epsilon; IL-1F9; IL-1H1
uniprot entry name :
IL36G_HUMAN
specificity :
No significant cross-reactivity or interference between Mouse IL1F9 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 pg/ml - 200 pg/ml. Sensitivity: 1.0 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL1F9 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL1F9 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL1F9 and IL1F9 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL1F9 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL1F9 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL1F9 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL1F9 and IL1F9 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL1F9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_062564.1
ncbi gb acc num :
NM_019618.3
ncbi mol weight :
18,721 Da
ncbi summary :
The protein encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]
uniprot summary :
Il1f9: Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response. Belongs to the IL-1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 2q12-q21. Cellular Component: extracellular space. Molecular Function: interleukin-1 receptor binding; cytokine activity. Biological Process: cell-cell signaling; cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of interleukin-6 production; negative regulation of myoblast differentiation; inflammatory response