catalog number :
MBS083256
products type :
ELISA Kit
products full name :
Mouse Microphthalmia Associated Transcription Factor ELISA Kit
products short name :
Microphthalmia Associated Transcription Factor
products gene name :
MITF
specificity :
No significant cross-reactivity or interference between Mouse MITF and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MITF concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse MITF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MITF and MITF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MITF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: The detection range of this kit is 0.25ng/ml-8ng/ml.
other info2 :
Sensitivity: The sensitivity of this kit is 0.1ng/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MITF concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse MITF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MITF and MITF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MITF in the samples is then determined by comparing the O.D. of the samples to the standard curve.