catalog number :
MBS083095
products type :
ELISA Kit
products full name :
Mouse Semaphorin 3A ELISA Kit
products short name :
Semaphorin 3A
products gene name :
SEMA3A
specificity :
No significant cross-reactivity or interference between Mouse SEMA3A and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 ng/ml - 200 ng/ml. Sensitivity: 1.0 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SEMA3A concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse SEMA3A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SEMA3A and SEMA3A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SEMA3A in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SEMA3A concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse SEMA3A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SEMA3A and SEMA3A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SEMA3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.