catalog number :
MBS082668
products type :
ELISA Kit
products full name :
Canine Insulin Like Growth Factor Binding Protein 7 ELISA Kit
products short name :
Insulin Like Growth Factor Binding Protein 7
products gene name :
IGFBP7
specificity :
No significant cross-reactivity or interference between Canine IGFBP7 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 6.25 pg/ml - 200 pg/ml. Sensitivity: 1.0 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IGFBP7 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine IGFBP7 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IGFBP7 and IGFBP7 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IGFBP7 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IGFBP7 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine IGFBP7 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IGFBP7 and IGFBP7 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IGFBP7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.