Fish Amylase Alpha ELISA Kit | MyBioSource MBS077098 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Fish Amylase Alpha ELISA Kit

catalog :

MBS077098

quantity :

48-Strip-Wells

price :

470 USD

product information

catalog number :

MBS077098

products type :

ELISA Kit

products full name :

Fish Amylase Alpha ELISA Kit

products short name :

[Amylase Alpha]

other names :

[amylase; Pancreatic alpha-amylase; pancreatic alpha-amylase; glycogenase; found in the pancreas; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase, pancreatic, alpha-2A; 1,4-alpha-D-glucan glucanohydrolase; amylase, alpha 2A (pancreatic); 1,4-alpha-D-glucan glucanohydrolase]

products gene name :

[Amyalpha]

other gene names :

[AMY2A; AMY2A; PA; AMY2; AMY2B; PA]

uniprot entry name :

AMYP_HUMAN

reactivity :

Fish

sequence length :

511

specificity :

No significant cross-reactivity or interference between Fish Amyalpha and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 15.6U/L-500U/L. Sensitivity: 2.0U/L.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated Amyalpha concentrations in Fish serum, plasma and other body fluids. Using Purified Fish Amyalpha antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add Amyalpha and Amyalpha antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Amyalpha in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated Amyalpha concentrations in Fish serum, plasma and other body fluids. Using Purified Fish Amyalpha antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add Amyalpha and Amyalpha antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Amyalpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

529397

ncbi acc num :

AAA51724.1

uniprot acc num :

P04746

ncbi mol weight :

57,707 Da

ncbi pathways :

Carbohydrate Digestion And Absorption Pathway (170720); Carbohydrate Digestion And Absorption Pathway (170654); Digestion Of Dietary Carbohydrate Pathway (106197); Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Metabolism Pathway (477135); Metabolism Of Carbohydrates Pathway (106196); Myoclonic Epilepsy Of Lafora Pathway (980469); Pancreatic Secretion Pathway (169306); Pancreatic Secretion Pathway (169295)

ncbi summary :

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

uniprot summary :

AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]. Protein type: Secreted; Secreted, signal peptide; EC 3.2.1.1; Hydrolase; Carbohydrate Metabolism - starch and sucrose. Chromosomal Location of Human Ortholog: 1p21. Cellular Component: extracellular space; extracellular region. Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding. Biological Process: polysaccharide digestion; carbohydrate catabolic process; carbohydrate metabolic process; pathogenesis

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

680

size3 :

5x96-Strip-Wells

price3 :

3100

size4 :

10x96-Strip-Wells

price4 :

6095