catalog number :
MBS076340
products type :
ELISA Kit
products full name :
Human Calpain 2, Large Subunit ELISA Kit
products short name :
[Calpain 2, Large Subunit]
other names :
[calpain-2 catalytic subunit isoform 2; Calpain-2 catalytic subunit; calpain-2 catalytic subunit; CANP 2; M-calpain; calpain M-type; millimolar-calpain; calpain-2 large subunit; calpain 2, large subunit; calpain large polypeptide L2; calpain, large polypeptide L2; calpain 2, large [catalytic] subunit; calcium-activated neutral proteinase 2; calpain 2, (m/II) large subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain]
products gene name :
[CAPN2]
other gene names :
[CAPN2; CAPN2; CANP2; mCANP; CANPL2; CANPml; CANPL2; CANP 2; M-calpain]
uniprot entry name :
CAN2_HUMAN
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Body fluids, tissue homogenates, secretions or feces samples. Assay Type: Quantitative Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of CAPN2 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_001139540.1
ncbi gb acc num :
NM_001146068.1
ncbi mol weight :
79,995 Da
ncbi pathways :
Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Apoptosis Pathway (83060); Apoptosis Pathway (470); ErbB1 Downstream Signaling Pathway (138057); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Integrin-mediated Cell Adhesion Pathway (198816); Protein Processing In Endoplasmic Reticulum Pathway (167325)
ncbi summary :
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
uniprot summary :
CAPN2: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Ubiquitous. Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin. Belongs to the peptidase C2 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.22.53; Protease; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q41-q42. Cellular Component: Golgi apparatus; cortical actin cytoskeleton; focal adhesion; endoplasmic reticulum; cytoplasm; dendrite; plasma membrane; pseudopodium; chromatin; cytosol; nucleus; lipid raft. Molecular Function: protein binding; protein heterodimerization activity; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; cysteine-type peptidase activity. Biological Process: protein autoprocessing; blastocyst development; proteolysis involved in cellular protein catabolic process; response to hypoxia; proteolysis; myoblast fusion; regulation of cytoskeleton organization and biogenesis
size4 :
10x96-Strip-Wells