ELISA/assay

Human Glucose-6-Phosphatase, Catalytic ELISA Kit

MBS070740

48-Strip-Wells

435 USD

ELISA Kit

Human Glucose-6-Phosphatase, Catalytic ELISA Kit

Glucose-6-Phosphatase, Catalytic

Glucose-6-phosphatase; Glucose-6-phosphatase; glucose-6-phosphatase; G6Pase; G-6-Pase; G6Pase-alpha; glucose-6-phosphatase alpha; glucose-6-phosphatase, catalytic subunit; Glucose-6-phosphatase alpha

G6PC

G6PC; G6PC; G6PT; GSD1; G6PC1; GSD1a; G6PT; G-6-Pase; G6Pase

G6PC_HUMAN

Human

357

No significant cross-reactivity or interference between Human G6PC and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 2.5 U/L - 80 U/L. Sensitivity: 0.5 U/L.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated G6PC concentrations in Human serum, plasma and other body fluids. Using Purified Human G6PC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add G6PC and G6PC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of G6PC in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated G6PC concentrations in Human serum, plasma and other body fluids. Using Purified Human G6PC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add G6PC and G6PC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of G6PC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

206729864

P35575.2

P35575

40,484 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Carbohydrate Digestion And Absorption Pathway (170720); Carbohydrate Digestion And Absorption Pathway (170654); DNA Damage Response (only ATM Dependent) Pathway (198827); Disease Pathway (530764); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); FoxO Family Signaling Pathway (138036)

Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]

G6PC: Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels. Defects in G6PC are the cause of glycogen storage disease type 1A (GSD1A). A metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. Belongs to the glucose-6-phosphatase family. Protein type: Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - starch and sucrose; Phosphatase (non-protein); Membrane protein, integral; Transporter; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 3.1.3.9; Transporter, SLC family; Membrane protein, multi-pass; Endoplasmic reticulum. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: endoplasmic reticulum membrane; integral to membrane; integral to endoplasmic reticulum membrane. Molecular Function: glucose-6-phosphatase activity; phosphotransferase activity, alcohol group as acceptor; phosphate binding. Biological Process: steroid metabolic process; glycogen metabolic process; response to food; phosphorylated carbohydrate dephosphorylation; glycogen catabolic process; multicellular organism growth; glucose 6-phosphate metabolic process; pathogenesis; glucose transport; glucose homeostasis; gluconeogenesis; cholesterol homeostasis; triacylglycerol metabolic process; urate metabolic process; regulation of gene expression; glucose-6-phosphate transport; hexose transport; carbohydrate metabolic process; transmembrane transport. Disease: Glycogen Storage Disease Ia

48-Strip-Wells

435 USD

96-Strip-Wells

600