ELISA/assay

Mouse Programmed Cell Death Protein 6 Interacting Protein ELISA Kit

MBS070738

48-Strip-Wells

435 USD

ELISA Kit

Mouse Programmed Cell Death Protein 6 Interacting Protein ELISA Kit

Programmed Cell Death Protein 6 Interacting Protein

programmed cell death 6 interacting protein, isoform CRA_b; Programmed cell death 6-interacting protein; programmed cell death 6-interacting protein; PDCD6-interacting protein; ALG-2 interacting protein 1; ALG-2-interacting protein X; dopamine receptor interacting protein 4; apoptosis-linked gene 2-interacting protein X; programmed cell death 6 interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95

PDCD6IP

PDCD6IP; PDCD6IP; AIP1; ALIX; HP95; DRIP4; AIP1; ALIX; KIAA1375; PDCD6-interacting protein

PDC6I_HUMAN

Mouse

751

No significant cross-reactivity or interference between Mouse PDCD6IP and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.5 ng/ml - 16 ng/ml. Sensitivity: 0.1 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PDCD6IP concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse PDCD6IP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PDCD6IP and PDCD6IP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PDCD6IP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PDCD6IP concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse PDCD6IP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PDCD6IP and PDCD6IP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PDCD6IP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119584873

EAW64469.1

Q8WUM4

96,023 Da

Budding And Maturation Of HIV Virion Pathway (106009); Disease Pathway (530764); Endocytosis Pathway (102279); Endocytosis Pathway (102181); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984); Late Phase Of HIV Life Cycle Pathway (105995); Uptake And Actions Of Bacterial Toxins Pathway (1016182); Uptake And Function Of Anthrax Toxins Pathway (1016190)

This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]

Alix: an adaptor protein that may regulate the function of receptor and cytoskeleton-associated tyrosine kinases. A class E vacuolar protein sorting (VPS) factor involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. May play a role in the regulation of both apoptosis and cell proliferation. Protein type: Adaptor/scaffold; Apoptosis. Chromosomal Location of Human Ortholog: 3p22.3. Cellular Component: focal adhesion; membrane; cytoplasm; melanosome; microtubule organizing center; immunological synapse; cytosol; vesicle. Molecular Function: protein binding; protein homodimerization activity; proteinase activated receptor binding; calcium-dependent protein binding; SH3 domain binding. Biological Process: protein transport; viral reproduction; cell separation during cytokinesis; apoptosis; viral infectious cycle; mitotic metaphase plate congression; nuclear organization and biogenesis

48-Strip-Wells

435 USD

96-Strip-Wells

600